Research Laboratory of the California State Department of Public Health, Berkeley.
J Exp Med. 1945 Oct 31;82(5):317-28. doi: 10.1084/jem.82.5.317.
Experiments to determine the optimum conditions for propagation of the virus of atypical pneumonia in chick embryos are described. Variations in the activity of infected chick embryo material were investigated. The highest dilution of chick embryo suspension producing pulmonary lesions in hamsters and cotton rats is not over 10(-3). Dilutions of 10(-4) infect chick embryos. The virus is unstable at room temperature and also loses activity when stored in a dry-ice refrigerator unless the suspensions are kept in sealed glass tubes. Filtration experiments indicate a maximum particle size of 180 to 250 mmicro. The virus propagated in chick embryos produces pulmonary lesions in hamsters and cotton rats which have been immunized to their own non-bacterial agents inducing pulmonary lesions. Of these, the pneumonia virus of hamsters most frequently causes intercurrent respiratory infections, and methods of controlling epizootics due to this agent are described.
描述了在鸡胚中繁殖非典型性肺炎病毒的最佳条件的实验。研究了受感染鸡胚材料的活性变化。在仓鼠和棉鼠中产生肺损伤的鸡胚悬液的最高稀释度不超过 10(-3)。10(-4) 的稀释度可感染鸡胚。病毒在室温下不稳定，如果不将悬浮液保存在密封的玻璃管中，在干冰冰箱中也会失去活性。过滤实验表明最大颗粒尺寸为 180 至 250 毫米。在鸡胚中繁殖的病毒会在已免疫其自身非细菌性致肺损伤剂的仓鼠和棉鼠中产生肺损伤。其中，仓鼠的肺炎病毒最常引起继发呼吸道感染，并描述了控制该病原体引起的传染病的方法。
