Hijiya N, Horiuchi K, Asakura T
Division of Hematology, Children's Hospital of Philadelphia, PA 19104.
J Lab Clin Med. 1991 Jan;117(1):60-6.
The effect of varying osmolarities (0.6% to 1.5% NaCl solutions, 213 to 492 mOsm/kg H2O) on the morphology of deoxygenated sickle cells was studied quantitatively with a computer-assisted image analysis system. Discocyte-rich, less dense fractions of sickle cells (density less than or equal to 1.11) were suspended in buffered NaCl solutions (pH 7.4) of various osmolarities, deoxygenated at room temperature for up to 5 hours, and stained by Wright's solution. Microscopic images were analyzed by circular shape factor (CSF = 4 pi x [area]/[perimeter]2) and elliptical shape factor (ESF = [short axis]/[long axis]). Since these two parameters yield different values for elongated cells and for cells of other shapes, such as maple-leaf- or star-shaped cells, the morphologic changes of sickle cells can be analyzed numerically. We found that both the rate and the degree of deformation depended highly on the osmotic pressure of the media in which the cells were suspended. In hypertonic solution, most sickle cells assumed a maple-leaf shape. The deformation occurred quickly, but the degree of deformation (circular shape factor and elliptical shape factor) was lower than that found in isotonic and slightly hypotonic solutions. Although elongated cells were formed in hypotonic and isotonic solutions, deformation was slower in these solutions than in hypertonic solutions. These results indicate that the shape and the degree of deformation of deoxygenated sickle cells are highly dependent on the osmolarity of the suspending medium and that the rate of deformation is inversely related to osmolarity. The relationship between morphology of deoxygenated sickle cells and osmotic pressure of the suspending media is discussed.
利用计算机辅助图像分析系统，定量研究了不同渗透压（0.6%至1.5%氯化钠溶液，213至492毫摩尔/千克水）对脱氧镰状细胞形态的影响。将富含圆盘状、密度较低的镰状细胞部分（密度小于或等于1.11）悬浮于不同渗透压的缓冲氯化钠溶液（pH 7.4）中，在室温下脱氧长达5小时，并用瑞氏染液染色。通过圆形形状因子（CSF = 4π×[面积]/[周长]²）和椭圆形形状因子（ESF = [短轴]/[长轴]）对显微镜图像进行分析。由于这两个参数对于细长细胞以及其他形状的细胞（如枫叶状或星形细胞）会产生不同的值，因此可以对镰状细胞的形态变化进行数值分析。我们发现，变形的速率和程度高度依赖于细胞所悬浮介质的渗透压。在高渗溶液中，大多数镰状细胞呈现枫叶状。变形迅速发生，但变形程度（圆形形状因子和椭圆形形状因子）低于等渗和轻度低渗溶液中的情况。虽然在低渗和等渗溶液中会形成细长细胞，但这些溶液中的变形比高渗溶液中慢。这些结果表明，脱氧镰状细胞的形状和变形程度高度依赖于悬浮介质的渗透压，并且变形速率与渗透压呈负相关。本文讨论了脱氧镰状细胞形态与悬浮介质渗透压之间的关系。
