巨大芽孢杆菌培养物中噬菌体和明胶酶浓度的增加。

INCREASE IN BACTERIOPHAGE AND GELATINASE CONCENTRATION IN CULTURES OF BACILLUS MEGATHERIUM.

Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.

J Gen Physiol. 1939 Sep 20;23(1):59-79. doi: 10.1085/jgp.23.1.59.

Abstract

The increase in bacteria, phage concentration, and gelatinase concentration in cultures of B. megatherium has been determined. 2. With lysogenic cultures the phage concentration, gelatinase concentration, and bacteria concentration increase logarithmically at first. The phage and gelatinase concentration then decrease while the bacteria concentration increases to a maximum. 3. The results are the same with sensitive cultures if the ratio of phage to bacteria is small. If the ratio of phage to bacteria is large phage, gelatinase, and bacteria concentration all increase at first and then decrease. The maximum rate of increase coincides approximately with the maximum rate of oxygen consumption of the culture. 4. 60-90 per cent of the phage is free from the cells. 5. The amount of phage produced is determined by the combined phage and not by the total phage. 6. Phage is produced during growth of the cells and not during lysis. 7. In a very narrow range of pH near 5.55 no increase in bacteria occurs but large increases in phage may be obtained.

摘要

已经确定了巨大芽孢杆菌培养物中细菌、噬菌体浓度和明胶酶浓度的增加。
在溶原性培养物中，噬菌体浓度、明胶酶浓度和细菌浓度最初呈对数增加。然后，噬菌体和明胶酶浓度下降，而细菌浓度增加到最大值。
如果噬菌体与细菌的比例较小，则敏感培养物的结果相同。如果噬菌体与细菌的比例较大，则噬菌体、明胶酶和细菌浓度最初都会增加，然后减少。最大增加速率与培养物的最大耗氧量大致吻合。
60-90%的噬菌体游离于细胞之外。
产生的噬菌体数量由结合噬菌体决定，而不是由总噬菌体决定。
噬菌体在细胞生长过程中产生，而不是在裂解过程中产生。
在 pH 值接近 5.55 的非常狭窄的范围内，细菌不会增加，但可能获得大量噬菌体的增加。