Section on Cellular Physiology, Laboratory of Biochemistry, National Heart Institute, National Institutes of Health, Bethesda, Maryland 20014.
J Gen Physiol. 1968 Jul 1;52(1):257-78.
The paucimolecular unit membrane model of the structure of the plasma membrane is critically reviewed in relation to current knowledge of the chemical and enzymatic composition of isolated plasma membranes, the properties of phospholipids, the chemistry of fixation for electron microscopy, the conformation of membrane proteins, the nature of the lipid-protein bonds in membranes, and possible mechanisms of transmembrane transport and membrane biosynthesis. It is concluded that the classical models, although not disproven, are not well supported by, and are difficult to reconcile with, the data now available. On the other hand, although a model based on lipoprotein subunits is, from a biochemical perspective, an attractive alternative, it too is far from proven. Many of the questions may be resolved by studies of membrane function and membrane biosynthesis rather than by a direct attack on membrane structure.
本文批判性地回顾了质膜结构的寡分子单位膜模型，涉及到当前对分离质膜的化学和酶组成、磷脂的性质、电子显微镜固定的化学、膜蛋白的构象、膜中脂质-蛋白质键的性质以及跨膜运输和膜生物合成的可能机制的认识。结论是，经典模型虽然没有被否定，但也没有得到现有数据的很好支持，而且很难与之协调。另一方面，虽然基于脂蛋白亚基的模型从生化角度来看是一个有吸引力的替代方案，但它也远未得到证实。许多问题可以通过对膜功能和膜生物合成的研究来解决，而不是直接对膜结构进行攻击。
