Gilloteaux J
Histochemistry. 1978 Apr 4;55(3):209-24. doi: 10.1007/BF00495760.
Specific and non specific cholinesterase activities were demonstrated in the ABRM of Mytilus edulis L. and Mytilus galloprovincialis L. by means of different techniques. The results were found identical for both species: neuromuscular junctions "en grappe"-type scarely distributed within the ABRM, contain AChE. According to the histochemical inhibition tests, (a) the eserine inhibits AChE activity of the ABRM with a level of 5-10(-5) M or higher, (b) the ChE non specific activities are inhibited by iso-OMPA level between 5.10(-5) to 10(-4) M. The histo- and cytochemical observations were completed by showing the existence of neuromuscular junctions containing small clear vesicles: they probably are the morphological support for ACh presence. Moreover, specific and non specific ChE activities were localized in the glio-interstitial cells. AChE precipitates were developed along the ABRM sarcolemma, some muscle mitochondria and in the intercellular spaces remain enigmatic.
运用不同技术在紫贻贝和地中海贻贝的副闭壳肌中证实了特异性和非特异性胆碱酯酶活性。结果发现这两个物种的情况相同:“葡萄状”神经肌肉接头在副闭壳肌内分布稀少,含有乙酰胆碱酯酶。根据组织化学抑制试验,(a)毒扁豆碱在5×10⁻⁵ M或更高浓度时抑制副闭壳肌的乙酰胆碱酯酶活性,(b)非特异性胆碱酯酶活性被异氟磷在5×10⁻⁵至10⁻⁴ M的浓度范围内抑制。通过显示含有小而清亮囊泡的神经肌肉接头的存在,完成了组织学和细胞化学观察:它们可能是乙酰胆碱存在的形态学支持。此外,特异性和非特异性胆碱酯酶活性定位于神经胶质间质细胞中。乙酰胆碱酯酶沉淀沿着副闭壳肌肌膜、一些肌肉线粒体以及细胞间隙形成,其情况仍不明朗。