Pomerantz R J, Feinberg M B, Andino R, Baltimore D
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
J Virol. 1991 Feb;65(2):1041-5. doi: 10.1128/JVI.65.2.1041-1045.1991.
The long terminal repeats (LTRs) of human immunodeficiency virus type 1 (HIV-1) strains from the central nervous systems of four patients with AIDS and of an HIV-1 isolate which is highly macrophage-tropic were isolated by using the polymerase chain reaction. In transient transfection assays, these LTRs demonstrated no significant difference in basal or stimulated levels of transcription in any of a variety of cell lines tested, compared with expression directed from the LTR of a T-lymphocyte-tropic strain of HIV-1. Chimeric viruses were created with the LTRs of the macrophage-tropic and brain-derived viruses ligated to the viral backbone from a T-lymphocyte-tropic strain. No change in cellular tropism was demonstrated with these chimeric viruses. Thus, unlike the LTRs of some murine retroviruses, the LTR of HIV-1 does not appear to play a major role in determining cellular tropism.
通过聚合酶链反应，从4例艾滋病患者中枢神经系统的人类免疫缺陷病毒1型（HIV-1）毒株以及一种高度嗜巨噬细胞的HIV-1分离株中分离出长末端重复序列（LTR）。在瞬时转染试验中，与HIV-1 T淋巴细胞嗜性毒株的LTR所指导的表达相比，这些LTR在任何一种受试细胞系中的基础转录水平或刺激转录水平均无显著差异。构建了嵌合病毒，将嗜巨噬细胞病毒和脑源病毒的LTR连接到T淋巴细胞嗜性毒株的病毒骨架上。这些嵌合病毒未表现出细胞嗜性的改变。因此，与某些鼠类逆转录病毒的LTR不同，HIV-1的LTR似乎在决定细胞嗜性方面不发挥主要作用。
