Laboratory of Immunology and Allergology, Istituto Dermopatico dell'Immacolata, Rome, Italy.
Clin Exp Dermatol. 2010 Oct;35(7):765-70. doi: 10.1111/j.1365-2230.2009.03694.x.
The basophil activation test (BAT) has been recently described as a useful in vitro tool for diagnosis of allergy to Anisakis species in patients with acute urticaria.
To evaluate the relationship between sensitization to Anisakis simplex and chronic urticaria (CU), using flow cytometry analysis of in vitro BAT. Methods. A. simplex sensitization was evaluated in patients with CU (n = 57) and in atopic (n = 22) and healthy controls (n = 20) by means of skin prick test (SPT), specific IgE and Anisakis-induced BAT using a triple-labelled strategy with anti-CD123, anti-human leucocyte antigen DR and anti-CD63 antibodies. During a follow-up period of 6 months in 10 patients with CU who accepted a fish-free dietary regimen, the diagnostic performance of the in vivo and in vitro methods was calculated, and changes in specific IgE and BAT were evaluated with respect to clinical response.
A significant association between CU and A. simplex sensitization was found, with an overall prevalence of 75.4% in patients with CU (43/57) compared with 18% (4/22) and 10% (2/20) of the atopic and healthy controls, respectively (P < 0.0001). BAT (cut-off > 13%) had the highest sensitivity and specificity, with significantly better ability than specific IgE testing for the identification of A. simplex sensitization in patients with CU. During the 6-month follow-up, clinical improvement was seen in all patients, and specific IgE and BAT results decreased to normal values in 6/10 (60%) and 10/10 (100%) patients, respectively.
BAT can be considered a reliable new in vitro method to evaluate A. simplex hypersensitivity in patients with CU, supplementing standardized procedures in both diagnosis and follow-up.
嗜碱性粒细胞活化试验（BAT）最近被描述为一种有用的体外工具，可用于诊断急性荨麻疹患者对旋毛虫属物种的过敏。
通过体外 BAT 的流式细胞术分析来评估对简单旋毛虫过敏与慢性荨麻疹（CU）之间的关系。方法。通过皮肤点刺试验（SPT）、特异性 IgE 和使用抗 CD123、抗人类白细胞抗原 DR 和抗 CD63 抗体的三标策略评估 A. simplex 致敏，在 CU（n=57）、特应性（n=22）和健康对照（n=20）患者中评估 A. simplex 致敏。在接受无鱼饮食方案的 10 例 CU 患者中进行了 6 个月的随访期，计算了体内和体外方法的诊断性能，并评估了特异性 IgE 和 BAT 随临床反应的变化。
发现 CU 与 A. simplex 致敏之间存在显著关联，CU 患者的总体患病率为 75.4%（43/57），而特应性和健康对照组分别为 18%（4/22）和 10%（2/20）（P<0.0001）。BAT（cut-off > 13%）具有最高的灵敏度和特异性，与特异性 IgE 检测相比，它具有更好的能力来识别 CU 患者中的 A. simplex 致敏。在 6 个月的随访期间，所有患者的临床症状均有所改善，6/10（60%）和 10/10（100%）患者的特异性 IgE 和 BAT 结果分别降至正常水平。
BAT 可以被认为是一种可靠的新的体外方法，用于评估 CU 患者对简单旋毛虫的过敏反应，补充诊断和随访的标准化程序。
