大剂量地塞米松对重症急性胰腺炎大鼠脾损伤的保护作用。
Protecting effects of a large dose of dexamethasone on spleen injury of rats with severe acute pancreatitis.
机构信息
Department of General Surgery, Hangzhou First People's Hospital, Zhejiang Province, China.
出版信息
J Gastroenterol Hepatol. 2010 Feb;25(2):302-8. doi: 10.1111/j.1440-1746.2009.05999.x. Epub 2009 Oct 27.
BACKGROUND AND AIMS
To explore the protecting effects and mechanisms of dexamethasone on spleen injury in rats with severe acute pancreatitis (SAP).
METHODS
The rats were randomly divided into a model control group, treated group and sham-operated group. The contents of plasma endotoxin, serum NO, phospholipase A(2) enzyme (PLA(2)) and endothelin-1 (ET-1) were determined. The mortality rate, pathological changes and changes of Bax and Bcl-2 protein expression levels and apoptotic indexes in the spleen of rats were observed in all groups, respectively, at 3, 6 and 12 h after operation.
RESULTS
Although the survival rate was significantly higher in the treated group than in the model control group, there was no significantly different between them (P > 0.05). The expression levels of Bax and Bcl-2 proteins and apoptotic indexes were significantly higher in the treated group than in the model control group at different time points (P < 0.05 or P < 0.01) while other blood indexes contents and pathological severity scores of spleen were significantly lower in the treated group than in the model control group (P < 0.05, P < 0.01 or P < 0.001).
CONCLUSION
Dexamethasone can protect spleen from injury during SAP mainly by reducing the content of inflammatory mediators in blood.
背景与目的
探讨地塞米松对重症急性胰腺炎（SAP）大鼠脾损伤的保护作用及其机制。
方法
将大鼠随机分为模型对照组、治疗组和假手术组。测定各组大鼠血浆内毒素、血清一氧化氮（NO）、磷脂酶 A2（PLA2）和内皮素-1（ET-1）含量，观察各组大鼠术后 3、6、12 h 的死亡率、病理学变化及脾组织 Bax 和 Bcl-2 蛋白表达水平和凋亡指数的变化。
结果
治疗组大鼠的存活率虽明显高于模型对照组，但差异无统计学意义（P > 0.05）。治疗组大鼠在不同时间点 Bax 和 Bcl-2 蛋白表达水平和凋亡指数明显高于模型对照组（P < 0.05 或 P < 0.01），而血液其他指标含量和脾组织病理学严重程度评分明显低于模型对照组（P < 0.05、P < 0.01 或 P < 0.001）。
结论
地塞米松可通过降低血液中炎症介质的含量来保护 SAP 大鼠的脾脏免受损伤。
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