白纹伊蚊含多聚腺苷酸信使核糖核酸的甲基化成分
Methylated constituents of Aedes albopictus poly (A)-containing messenger RNA.
作者信息
HsuChen C C, Dubin D T
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2671-82. doi: 10.1093/nar/4.8.2671.
Abstract
Poly (A)-containing mRNA prepared from cultured mosquito (Aedes albopictus) cells was found to contain methylated 5'-terminal "caps" as well as internal m6A residues. Both type I [m7G(5')ppp(5')Xmp] and type II [m7G(5')ppp(5')XmpYmp] caps were present, at molar ratio of ca five to one. All four common RNA bases were represented in the second position (Xm) of the caps, adenine being the most abundant and N6-methyladenine being absent. The four bases were also represented in the third position (Ym), but here uracil was the predominant base. There was approximately one internal m6A residue for every three caps. These studies demonstrate that mRNA from an invertebrate source can have a methylation pattern comparable with that of mammalian cells in it complexity.
摘要
从培养的蚊子（白纹伊蚊）细胞中制备的含聚腺苷酸（Poly (A)）的信使核糖核酸（mRNA）被发现含有甲基化的5'-末端“帽”结构以及内部的N6-甲基腺嘌呤（m6A）残基。I型[m7G(5')ppp(5')Xmp]和II型[m7G(5')ppp(5')XmpYmp]帽结构均存在，摩尔比约为五比一。帽结构的第二个位置（Xm）包含所有四种常见的RNA碱基，腺嘌呤最为丰富，且不存在N6-甲基腺嘌呤。这四种碱基在第三个位置（Ym）也有体现，但此处尿嘧啶是主要碱基。每三个帽结构大约有一个内部m6A残基。这些研究表明，来自无脊椎动物的mRNA在甲基化模式的复杂性上可与哺乳动物细胞相媲美。
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