来自培养的仓鼠细胞的含聚腺苷酸信使核糖核酸的甲基化状态。
The methylation state of poly A-containing messenger RNA from cultured hamster cells.
作者信息
Dubin D T, Taylor R H
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1653-68. doi: 10.1093/nar/2.10.1653.
Abstract
The poly A-containing mRNA of cultured hamster (BHK-21) cells has been examined with regard to methylation status. Steady state-labeled mRNA was obtained by incubating cells for 20-22h in the presence of [methyl-3H]-methionine and 32Pi. The degree of methylation of this RNA was 1.8 methyl groups per 1000 nucleotides, or 4-5 methyl groups on the average per molecule. The nature of the methylated residues was determined by paper chromatography and electrophoresis of acid and alkaline hydrolysates, by DEAE cellulose chromatography of alkaline hydrolysates and of T2 RNase digests, and by examining the effect of subjecting samples to "beta-elimination." Approx. half of the methyl groups occurred in standard ("internal") linkage, 10% as m5Cp and 40% as m6Ap residues. The remainder occurred at least for the most part in "blocked" 5'-termini with the presumptive structure m7G(5')ppp(Nm)p.., where Nm was Gm, m6Am, Um, or Cm.
摘要
已对培养的仓鼠（BHK - 21）细胞中含多聚腺苷酸的mRNA的甲基化状态进行了研究。通过在[甲基 - ³H] - 甲硫氨酸和³²Pi存在下将细胞孵育20 - 22小时来获得稳态标记的mRNA。这种RNA的甲基化程度为每1000个核苷酸1.8个甲基，即平均每个分子有4 - 5个甲基。通过对酸性和碱性水解产物进行纸层析和电泳、对碱性水解产物和T2核糖核酸酶消化产物进行DEAE纤维素层析以及通过检测样品进行“β-消除”的效果来确定甲基化残基的性质。大约一半的甲基以标准（“内部”）连接形式存在，10%为m⁵Cp，40%为m⁶Ap残基。其余的至少大部分存在于具有推定结构m⁷G(5')ppp(Nm)p..的“封闭”5'-末端，其中Nm为Gm、m⁶Am、Um或Cm。
相似文献
1
The methylation state of poly A-containing messenger RNA from cultured hamster cells.来自培养的仓鼠细胞的含聚腺苷酸信使核糖核酸的甲基化状态。
Nucleic Acids Res. 1975 Oct;2(10):1653-68. doi: 10.1093/nar/2.10.1653.
2
5'-Terminal and internal methylated nucleotide sequences in HeLa cell mRNA.海拉细胞信使核糖核酸中的5'-末端和内部甲基化核苷酸序列。
Biochemistry. 1976 Jan 27;15(2):397-401. doi: 10.1021/bi00647a024.
3
Multiple methylated cap sequences in adenovirus type 2 early mRNA.腺病毒2型早期信使核糖核酸中的多个甲基化帽序列。
J Virol. 1976 Nov;20(2):425-35. doi: 10.1128/JVI.20.2.425-435.1976.
4
The 5'-terminal structures of murine alpha- and beta-globin messenger RNA.小鼠α-和β-珠蛋白信使核糖核酸的5'-末端结构
J Biol Chem. 1977 Mar 10;252(5):1758-63.
5
Methylated messenger RNA in mouse kidney.小鼠肾脏中的甲基化信使核糖核酸
Biochemistry. 1975 Oct 7;14(20):4361-67. doi: 10.1021/bi00691a003.
6
Methyl labeling of HeLa cell hnRNA: a comparison with mRNA.HeLa细胞核内不均一RNA的甲基化标记：与信使RNA的比较。
Cell. 1976 Feb;7(2):227-37. doi: 10.1016/0092-8674(76)90022-2.
7
Methylated nucleotides block 5' terminus of HeLa cell messenger RNA.甲基化核苷酸阻断了海拉细胞信使核糖核酸的5'末端。
Cell. 1975 Apr;4(4):379-86. doi: 10.1016/0092-8674(75)90158-0.
8
Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus.诺维科夫肝癌mRNA甲基化及甲基化5'末端异质性的表征
Biochemistry. 1975 Oct 7;14(20):4367-74. doi: 10.1021/bi00691a004.
9
Methylated and blocked 5' termini in vesicular stomatitis virus in vivo mRNAs.水泡性口炎病毒体内mRNA中的甲基化和封闭5'末端
Cell. 1975 May;5(1):59-67. doi: 10.1016/0092-8674(75)90092-6.
10
Methylated, blocked 5 termini in HeLa cell mRNA.海拉细胞信使核糖核酸中甲基化、封闭的5'末端
Proc Natl Acad Sci U S A. 1975 May;72(5):1904-8. doi: 10.1073/pnas.72.5.1904.
引用本文的文献
1
Bisulfite Sequencing to Map 5-Methylcytosine Sites in Human Respiratory Syncytial Virus RNA.亚硫酸氢盐测序法绘制人呼吸道合胞病毒RNA中的5-甲基胞嘧啶位点
Methods Mol Biol. 2025;2948:233-247. doi: 10.1007/978-1-0716-4666-3_15.
2
The role of m5C, m1A and m7G modifications in tumors of urinary system.m5C、m1A和m7G修饰在泌尿系统肿瘤中的作用。
Front Cell Dev Biol. 2025 Jul 30;13:1549588. doi: 10.3389/fcell.2025.1549588. eCollection 2025.
3
Targeting epigenetic regulators as a promising avenue to overcome cancer therapy resistance.将表观遗传调节因子作为克服癌症治疗耐药性的一条有前景的途径。
Signal Transduct Target Ther. 2025 Jul 18;10(1):219. doi: 10.1038/s41392-025-02266-z.
4
N6-Methyladenosine Promotes the Transcription of c-Src Kinase via IRF1 to Facilitate the Proliferation of Liver Cancer.N6-甲基腺苷通过IRF1促进c-Src激酶转录以促进肝癌增殖。
Oncol Res. 2025 Jun 26;33(7):1679-1693. doi: 10.32604/or.2025.062747. eCollection 2025.
5
Epitranscriptomic Role of m6A in Obesity-Associated Disorders and Cancer Metabolic Reprogramming.m6A的表观转录组学作用在肥胖相关疾病和癌症代谢重编程中
Genes (Basel). 2025 Apr 27;16(5):498. doi: 10.3390/genes16050498.
6
NSUN2 contributes to the RB malignant progression and Glycolysis by mediating the m5C methylation modification of HKDC1.NSUN2通过介导HKDC1的m5C甲基化修饰促进RB的恶性进展和糖酵解。
J Bioenerg Biomembr. 2025 May 27. doi: 10.1007/s10863-025-10062-1.
7
The regulatory role of m6A in cancer metastasis.m6A在癌症转移中的调控作用。
Front Cell Dev Biol. 2025 Apr 28;13:1539678. doi: 10.3389/fcell.2025.1539678. eCollection 2025.
8
Unveiling the potential impact of RNA m5C methyltransferases NSUN2 and NSUN6 on cellular aging.揭示RNA m5C甲基转移酶NSUN2和NSUN6对细胞衰老的潜在影响。
Front Genet. 2025 Apr 16;16:1477542. doi: 10.3389/fgene.2025.1477542. eCollection 2025.
9
N7-methylguanosine modification in cancers: from mechanisms to therapeutic potential.癌症中的N7-甲基鸟苷修饰：从机制到治疗潜力
J Hematol Oncol. 2025 Jan 29;18(1):12. doi: 10.1186/s13045-025-01665-7.
10
N6-methyladenosine (m6A) modification in inflammation: a bibliometric analysis and literature review.炎症中的N6-甲基腺苷（m6A）修饰：文献计量分析与文献综述
PeerJ. 2024 Dec 13;12:e18645. doi: 10.7717/peerj.18645. eCollection 2024.
本文引用的文献
1
The occurrence and distribution of thymine and three methylated-adenine bases in ribonucleic acids from several sources.几种来源的核糖核酸中胸腺嘧啶及三种甲基化腺嘌呤碱基的出现情况和分布
Biochem J. 1958 Dec;70(4):642-51. doi: 10.1042/bj0700642.
2
Messenger RNA turnover in mouse L cells.小鼠L细胞中的信使核糖核酸周转
J Mol Biol. 1973 Oct 5;79(4):681-96. doi: 10.1016/0022-2836(73)90071-5.
3
Messenger RNA in HeLa cells: kinetics of formation and decay.海拉细胞中的信使核糖核酸：合成与降解动力学
J Mol Biol. 1973 Aug 5;78(2):321-34. doi: 10.1016/0022-2836(73)90119-8.
4
In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase.病毒体相关甲基转移酶对新生呼肠孤病毒mRNA的体外甲基化作用。
Nucleic Acids Res. 1974 Dec;1(12):1739-52. doi: 10.1093/nar/1.12.1739.
5
The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors.海拉细胞核糖体RNA及其前体中的甲基化核苷酸序列。
J Mol Biol. 1974 Sep 5;88(1):133-52. doi: 10.1016/0022-2836(74)90299-x.
6
Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing.信使核糖核酸5'末端含有甲基化核苷酸的序列：对加工过程的潜在影响。
Cell. 1974 Nov;3(3):197-9. doi: 10.1016/0092-8674(74)90131-7.
7
Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells.诺维科夫肝癌细胞信使核糖核酸中甲基化核苷的鉴定
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3971-5. doi: 10.1073/pnas.71.10.3971.
8
Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase.水泡性口炎病毒病毒粒子转录酶对RNA转录的起始与指导
J Virol. 1973 Apr;11(4):487-501. doi: 10.1128/JVI.11.4.487-501.1973.
9
Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s species.来自培养动物细胞的线粒体RNA。独特的高分子量和4s种类。
J Mol Biol. 1970 Mar 14;48(2):279-95. doi: 10.1016/0022-2836(70)90161-0.
10
The messenger-like properties of the poly(A)plus RNA in mammalian mitochondria.哺乳动物线粒体中多聚腺苷酸（poly(A)）加尾RNA的信使样特性。
Cell. 1974 Dec;3(4):335-9. doi: 10.1016/0092-8674(74)90047-6.
Zotero 插件