Department of Biology, University of Dayton, Dayton, OH 45469-2320, USA.
Sci Total Environ. 2009 Dec 20;408(2):439-43. doi: 10.1016/j.scitotenv.2009.10.008. Epub 2009 Oct 29.
The growth of the nanotechnology industry and subsequent proliferation of nanoparticle types present the need to rapidly assess nanoparticle toxicity. We present a novel, simple and cost-effective nebulizer-based method to deliver nanoparticles to the Drosophila melanogaster respiratory system, for the purpose of toxicity testing. FluoSpheres, silver, and CdSe/ZnS nanoparticles of different sizes were effectively aerosolized, showing the system is capable of functioning with a wide range of nanoparticle types and sizes. Red fluorescent CdSe/ZnS nanoparticles were successfully delivered to the fly respiratory system, as visualized by fluorescent microscopy. Silver coated and uncoated nanoparticles were delivered in a toxicity test, and induced Hsp70 expression in flies, confirming the utility of this model in toxicity testing. This is the first method developed capable of such delivery, provides the advantage of the Drosophila health model, and can serve as a link between tissue culture and more expensive mammalian models in a tiered toxicity testing strategy.
纳米技术产业的发展和随后的纳米颗粒类型的增殖，需要快速评估纳米颗粒的毒性。我们提出了一种新颖、简单和具有成本效益的基于喷雾器的方法，将纳米颗粒递送到黑腹果蝇的呼吸系统，用于毒性测试。FluoSpheres、银和不同尺寸的 CdSe/ZnS 纳米颗粒被有效地雾化，表明该系统能够与多种类型和尺寸的纳米颗粒一起工作。通过荧光显微镜观察到红色荧光 CdSe/ZnS 纳米颗粒成功递送到果蝇的呼吸系统。在毒性测试中，递送了银涂层和未涂层的纳米颗粒，并诱导了果蝇中 Hsp70 的表达，证实了该模型在毒性测试中的实用性。这是第一个能够进行这种递送的方法，提供了黑腹果蝇健康模型的优势，并可以在分层毒性测试策略中作为组织培养和更昂贵的哺乳动物模型之间的联系。
