Department of Ophthalmology, University of Rostock, Rostock, Germany.
J Cataract Refract Surg. 2009 Nov;35(11):1979-83. doi: 10.1016/j.jcrs.2009.06.019.
To evaluate a new method for visualizing femtosecond laser pulse-induced microincisions inside crystalline lens tissue.
Laser Zentrum Hannover e.V., Hannover, Germany.
Lenses removed from porcine eyes were modified ex vivo by femtosecond laser pulses (wavelength 1040 nm, pulse duration 306 femtoseconds, pulse energy 1.0 to 2.5 microJ, repetition rate 100 kHz) to create defined planes at which lens fibers separate. The femtosecond laser pulses were delivered by a 3-dimension (3-D) scanning unit and transmitted by focusing optics (numerical aperture 0.18) into the lens tissue. Lens fiber orientation and femtosecond laser-induced microincisions were examined using a confocal laser scanning microscope (CLSM) based on a Rostock Cornea Module attached to a Heidelberg Retina Tomograph II. Optical sections were analyzed in 3-D using Amira software (version 4.1.1).
Normal lens fibers showed a parallel pattern with diameters between 3 microm and 9 microm, depending on scanning location. Microincision visualization showed different cutting effects depending on pulse energy of the femtosecond laser. The effects ranged from altered tissue-scattering properties with all fibers intact to definite fiber separation by a wide gap. Pulse energies that were too high or overlapped too tightly produced an incomplete cutting plane due to extensive microbubble generation.
The 3-D CLSM method permitted visualization and analysis of femtosecond laser pulse-induced microincisions inside crystalline lens tissue. Thus, 3-D CLSM may help optimize femtosecond laser-based procedures in the treatment of presbyopia.
评估一种可视化飞秒激光脉冲在晶状体组织内诱导的微切口的新方法。
德国汉诺威激光中心。
从猪眼取出的晶状体经飞秒激光脉冲（波长 1040nm，脉冲持续时间 306fs，脉冲能量 1.0 至 2.5μJ，重复频率 100kHz）进行体外修饰，以在晶状体纤维分离的特定平面上进行修改。飞秒激光脉冲由 3 维（3-D）扫描单元传输，并通过聚焦光学元件（数值孔径 0.18）传输到晶状体组织中。使用基于附加于海德堡视网膜断层扫描仪 II 的 Rostock 角膜模块的共聚焦激光扫描显微镜（CLSM）检查晶状体纤维取向和飞秒激光诱导的微切口。使用版本 4.1.1 的 Amira 软件对光学截面进行 3-D 分析。
正常晶状体纤维呈现出直径在 3μm 至 9μm 之间的平行模式，具体取决于扫描位置。微切口可视化显示，飞秒激光的脉冲能量不同，切割效果也不同。效果范围从所有纤维完整的组织散射特性改变到通过宽间隙的明确纤维分离。由于产生了广泛的微泡，能量过高或重叠过紧的脉冲会产生不完整的切割平面。
3-D CLSM 方法允许可视化和分析晶状体组织内的飞秒激光脉冲诱导的微切口。因此，3-D CLSM 可能有助于优化基于飞秒激光的老视治疗程序。
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