Sampi K, Takagi T, Sakai C, Kuraishi Y, Ishige K
Saitama Cancer Center, Hematology Clinic, Japan.
Gan To Kagaku Ryoho. 1991 Jan;18(1):69-73.
An improved method for the detection of thymidine kinase (TK) activity with the use of 125I-iododeoxyuridine as the substrate. Radioimmunoassay of prolifigen TK "Daiichi", was used for this assay. Eighty-seven serum samples were collected from 40 patients with acute nonlymphocytic leukemia in four institutions. The levels of TK were measured in the time of pretreatment, remission, and recurrence, and the relationship between the levels of TK and either LDH, marrow blasts, or circulating blasts was also examined. The levels of TK were significantly lower in the state of remission than in the pretreatment. However, the level of TK in remission was much elevated in the majority of cases than normal range (less than or equal to 5 units/l). On the contrary, LDH in remission was within normal limits in the majority of cases. The level of TK in the state of relapse was significantly higher than in the remission. The level of TK correlated in some extent with the percentage of marrow blasts (r = 0.508), and the level of TK in more than 5% of marrow blast was most beyond normal range. Correlation coefficient between the percentage of circulating blasts and TK (r = 0.577) was slightly higher than that between the percentage of marrow blasts and TK. Correlation coefficient between the level of TK and the number of marrow blasts was higher than that between LDH and number of marrow blasts. The levels of TK correlated well with serum LDH (r = 0.778), and was more sensitive than LDH. In conclusion, it was suggested that TK could be used as one of an useful and supplemental tumor marker in the follow-up of treatment of acute nonlymphocytic leukemia and to monitor the effect of therapy.
一种以¹²⁵I-碘脱氧尿苷为底物检测胸苷激酶（TK）活性的改进方法。本检测采用了增殖型TK“第一制药”的放射免疫分析。从四个机构的40例急性非淋巴细胞白血病患者中收集了87份血清样本。在预处理、缓解和复发时测量TK水平，并检查TK水平与乳酸脱氢酶（LDH）、骨髓原始细胞或循环原始细胞之间的关系。缓解状态下的TK水平显著低于预处理时。然而，大多数病例缓解期的TK水平比正常范围（小于或等于5单位/升）升高很多。相反，大多数病例缓解期的LDH在正常范围内。复发状态下的TK水平显著高于缓解期。TK水平在一定程度上与骨髓原始细胞百分比相关（r = 0.508），骨髓原始细胞超过5%时的TK水平大多超出正常范围。循环原始细胞百分比与TK之间的相关系数（r = 0.577）略高于骨髓原始细胞百分比与TK之间的相关系数。TK水平与骨髓原始细胞数量的相关系数高于LDH与骨髓原始细胞数量的相关系数。TK水平与血清LDH相关性良好（r = 0.778），且比LDH更敏感。总之，提示TK可作为急性非淋巴细胞白血病治疗随访及监测治疗效果的有用且补充性的肿瘤标志物之一。
