儿茶酚胺敏感腺苷酸环化酶活性的重建：可溶性成分与富含受体的膜之间的相互作用
Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.
作者信息
Ross E M, Gilman A G
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3715-9. doi: 10.1073/pnas.74.9.3715.
Membranes of mouse L cells that contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] but lack beta-adrenergic receptors have been solubilized with Lubrol 12A9. Addition of such adenylate cyclase-containing extracts to beta-adrenergic receptor-replete membranes from adenylate cyclase-deficient S49 lymphoma cells results in the production of a catecholamine-sensitive adenylate cyclase system. The effects of beta-adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild-type S49 lymphoma cell. The uncoupled variant of the S49lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone-sensitive enzyme activity when added to adenylate cyclase-deficient membranes. Thus, the uncoupled and adenylate cyclase-deficient variants of the S49 cell are not complementary.
含有腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]但缺乏β-肾上腺素能受体的小鼠L细胞的膜已用Lubrol 12A9溶解。将这种含腺苷酸环化酶的提取物添加到来自腺苷酸环化酶缺陷型S49淋巴瘤细胞的富含β-肾上腺素能受体的膜中，会产生一种对儿茶酚胺敏感的腺苷酸环化酶系统。β-肾上腺素能激动剂和拮抗剂对重构系统的作用再现了野生型S49淋巴瘤细胞的特征。S49淋巴瘤的解偶联变体含有腺苷酸环化酶，但当将该克隆的供体提取物添加到腺苷酸环化酶缺陷型膜中时，无法重构激素敏感的酶活性。因此，S49细胞的解偶联和腺苷酸环化酶缺陷变体不具有互补性。
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