Plant Science Education Unit, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101, Japan. f
Plant Cell Physiol. 2009 Dec;50(12):2234-9. doi: 10.1093/pcp/pcp154.
Zinc (Zn) is an essential micronutrient for various physiological and metabolic processes in plants, although it is toxic in excess. To understand better Zn-responsive proteins, we developed a highly improved method of isoelectric focusing (IEF) in which whole lysate from Arabidopsis roots is subjected to IEF without any desalting steps. In this method, samples extracted with lysis buffer containing 1.5% SDS can also be directly applied to IEF. By applying this method to Zn-treated Arabidopsis roots, 10 up-regulated and 17 down-regulated proteins were identified, 15 of which showed a significant correlation with previously reported transcriptomic data.
锌（Zn）是植物各种生理和代谢过程所必需的微量元素，但过量则具有毒性。为了更好地了解锌响应蛋白，我们开发了一种高度改良的等电聚焦（IEF）方法，该方法无需任何脱盐步骤，可对来自拟南芥根的全裂解物进行 IEF。在该方法中，用含有 1.5%SDS 的裂解缓冲液提取的样品也可以直接应用于 IEF。通过将该方法应用于经锌处理的拟南芥根，鉴定出 10 种上调和 17 种下调蛋白，其中 15 种与先前报道的转录组数据具有显著相关性。
