Deutscher Murray P
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida, USA.
Methods Enzymol. 2009;463:343-5. doi: 10.1016/S0076-6879(09)63021-4.
Passage of an extract or supernatant fraction through a column of Affi-Gel Blue and batchwise elution can be a rapid and effective early procedure for removal of nucleic acid, concentration of the sample and purification of nucleotide binding proteins.
将提取物或上清液组分通过Affi-Gel Blue柱并进行分批洗脱,对于去除核酸、浓缩样品以及纯化核苷酸结合蛋白而言,可能是一种快速有效的早期步骤。