Charite-Universitatsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Skin Cancer Center, Berlin, 10117, Germany.
J Biomed Opt. 2009 Sep-Oct;14(5):054001. doi: 10.1117/1.3213603.
Epidermal wound healing is a complex and dynamic regenerative process necessary to reestablish skin integrity. Fluorescence confocal laser scanning microscopy (FLSM) is a noninvasive imaging technique that has previously been used for evaluation of inflammatory and neoplastic skin disorders in vivo and at high resolution. We employed FLSM to investigate the evolution of epidermal wound healing noninvasively over time and in vivo. Two suction blisters were induced on the volar forearms of the study participants, followed by removal of the epidermis. To study the impact of wound ointment on the process of reepithelization, test sites were divided into two groups, of which one test site was left untreated as a negative control. FLSM was used for serial/consecutive evaluations up to 8 days. FLSM was able to visualize the development of thin keratinocyte layers developing near the wound edge and around hair follicles until the entire epidermis has been reestablished. Wounds treated with the wound ointment were found to heal significantly faster than untreated wounds. This technique allows monitoring of the kinetics of wound healing noninvasively and over time, while offering new insights into the potential effects of topically applied drugs on the process of tissue repair.
表皮伤口愈合是一个复杂而动态的再生过程，对于重建皮肤完整性是必要的。荧光共聚焦激光扫描显微镜（FLSM）是一种非侵入性的成像技术，之前已被用于体内和高分辨率评估炎症和肿瘤性皮肤疾病。我们采用 FLSM 来研究表皮伤口愈合在体内随时间的演变。在研究参与者的掌前区上诱导两个抽吸水疱，然后去除表皮。为了研究伤口软膏对再上皮化过程的影响，将试验部位分为两组，其中一个试验部位作为阴性对照未进行处理。FLSM 用于连续评估，最长可达 8 天。FLSM 能够可视化在伤口边缘附近和毛囊周围形成的薄角蛋白细胞层的发展，直到整个表皮都已重建。用伤口软膏处理的伤口被发现比未处理的伤口愈合得更快。该技术允许在体内和随时间对伤口愈合的动力学进行非侵入性监测，并为局部应用药物对组织修复过程的潜在影响提供新的见解。
