Charité University Medicine Berlin, Department of Dermatology, Skin Cancer Center Charité, Universitätsmedizin Berlin, Charité Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
J Biomed Opt. 2012 Jul;17(7):076016. doi: 10.1117/1.JBO.17.7.076016.
There is a high demand for noninvasive imaging techniques for wound assessment. In vivo reflectance confocal laser scanning microscopy (CLSM) represents an innovative optical technique for noninvasive evaluation of normal and diseased skin in vivo at near cellular resolution. This study was designed to test the feasibility of CLSM for noninvasive analysis of cutaneous wound healing in 15 patients (7 male/8 female), including acute and chronic, superficial and deep dermal skin wounds. A commercially available CLSM system was used for the assessment of wound bed and wound margins in order to obtain descriptive cellular and morphological parameters of cutaneous wound repair noninvasively and over time. CLSM was able to visualize features of cutaneous wound repair in epidermal and superficial dermal wounds, including aspects of inflammation, neovascularisation, and tissue remodelling in vivo. Limitations include the lack of mechanic fixation of the optical system on moist surfaces restricting the analysis of chronic skin wounds to the wound margins, as well as a limited optical resolution in areas of significant slough formation. By describing CLSM features of cutaneous inflammation, vascularisation, and epithelialisation, the findings of this study support the role of CLSM in modern wound research and management.
对于用于伤口评估的非侵入性成像技术有很高的需求。体内反射共焦激光扫描显微镜 (CLSM) 代表了一种创新的光学技术，可在接近细胞分辨率的情况下对正常和患病皮肤进行非侵入性体内评估。本研究旨在测试 CLSM 对 15 名患者（7 名男性/8 名女性）的皮肤伤口愈合的非侵入性分析的可行性，包括急性和慢性、浅层和深层真皮皮肤伤口。使用市售的 CLSM 系统评估伤口床和伤口边缘，以便非侵入性和随时间推移获得皮肤伤口修复的描述性细胞和形态学参数。CLSM 能够可视化表皮和浅层真皮伤口修复的特征，包括体内炎症、新生血管形成和组织重塑的各个方面。局限性包括缺乏光学系统在潮湿表面上的机械固定，这限制了对慢性皮肤伤口的分析仅限于伤口边缘，以及在明显坏死组织形成的区域中光学分辨率有限。通过描述 CLSM 在皮肤炎症、血管生成和上皮形成方面的特征，本研究的结果支持 CLSM 在现代伤口研究和管理中的作用。
