Concordia University, Department of Mechanical Engineering, Optical-Bio Microsystems Laboratory, 1515 Street Catherine Ouest, Montreal, Quebec H3G 2W1, Canada.
J Biomed Opt. 2009 Sep-Oct;14(5):054050. doi: 10.1117/1.3210766.
The advent of microoptoelectromechanical systems (MOEMS) and its integration with other technologies such as microfluidics, microthermal, immunoproteomics, etc. has led to the concept of an integrated micro-total-analysis systems (microTAS) or Lab-on-a-Chip for chemical and biological applications. Recently, research and development of microTAS have attained a significant growth rate over several biodetection sciences, in situ medical diagnoses, and point-of-care testing applications. However, it is essential to develop suitable biophysical label-free detection methods for the success, reliability, and ease of use of the microTAS. We proposed an infrared (IR)-based evanescence wave detection system on the silicon-on-insulator platform for biodetection with microTAS. The system operates on the principle of bio-optical interaction that occurs due to the evanescence of light from the waveguide device. The feasibility of biodetection has been experimentally investigated by the detection of horse radish peroxidase upon its reaction with hydrogen peroxide.
微机电系统（MOEMS）的出现及其与微流控、微热、免疫蛋白质组学等其他技术的集成，催生了集成微全分析系统（microTAS）或芯片实验室的概念，用于化学和生物应用。最近，微 TAS 在多个生物检测科学、现场医学诊断和即时检测应用方面的研究和开发取得了显著的增长率。然而，开发合适的生物物理无标记检测方法对于微 TAS 的成功、可靠性和易用性至关重要。我们提出了一种基于硅绝缘体平台的基于红外（IR）的消逝波检测系统，用于微 TAS 的生物检测。该系统基于光在波导器件中消逝时发生的生物光学相互作用原理运行。通过检测辣根过氧化物酶与过氧化氢反应时的情况，实验研究了生物检测的可行性。
