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[急性肺炎尸检材料的免疫荧光研究方法]

[Immunofluorescent method of studying autopsy material in acute pneumonia].

作者信息

Kaplan A S, Andreeva T V

出版信息

Vopr Virusol. 1977(1):95-7.

PMID:198964
Abstract

The immunofluorescent procedure in examinations of the autopsy material from 304 fatal cases hospitalized for acute pneumonia permitted to diagnose influenza A2 in 29.0%, influenza B in 18.1%, parainfluenza in 3.5%, adenovirus infection in 9.2% and respiratory syncytial virus infection in 3.5% of the cases. In the period of a high incidence of acute respiratory infection, influenza A2 was detected by this method in 40.9% and influenza B in 50% of the cases. Simultaneous examinations of the material in the influenza epidemic period by virological and immunofluorescent methods (63 cases) in 13 cases positive results were obtained with both methods, in 6 cases where influenza viruses were detected the immunofluorescent test was negative, and in 28 cases the positive diagnosis by the immunofluorescent test could not be confirmed virologically. Among the cases examined, 33 were found by the immunofluorescence test to have a mixed respiratory infection, including influenza A2 with other forms of respiratory infection in 18, and influenza B with other respiratory infections in 19 cases. Serological examinations by the complement fixation and hemagglutination inhibition tests on the blood from fatal cases irrespective of the time of examination, as a rule, revealed antibody in low titres which did not confirm the diagnosis.

摘要

对304例因急性肺炎住院死亡病例的尸检材料进行免疫荧光检查,结果显示,29.0%的病例诊断为甲型流感2型,18.1%为乙型流感,3.5%为副流感,9.2%为腺病毒感染,3.5%为呼吸道合胞病毒感染。在急性呼吸道感染高发期,用该方法检测出40.9%的病例感染甲型流感2型,50%感染乙型流感。在流感流行期间,对材料同时采用病毒学和免疫荧光方法进行检测(63例),两种方法均呈阳性的有13例,检测出流感病毒但免疫荧光试验呈阴性的有6例,免疫荧光试验呈阳性但病毒学无法确诊的有28例。在所检查的病例中,通过免疫荧光试验发现33例有混合性呼吸道感染,其中18例为甲型流感2型合并其他形式的呼吸道感染,19例为乙型流感合并其他呼吸道感染。对死亡病例的血液,无论检查时间如何,常规采用补体结合试验和血凝抑制试验进行血清学检查,结果显示抗体滴度较低,无法确诊。

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