妊娠区带蛋白的一级结构。通过聚合酶链反应对全长妊娠区带蛋白cDNA克隆进行分子克隆。

Primary structure of pregnancy zone protein. Molecular cloning of a full-length PZP cDNA clone by the polymerase chain reaction.

作者信息

Devriendt K, Van den Berghe H, Cassiman J J, Marynen P

机构信息

Center for Human Genetics, University of Leuven, Belgium.

出版信息

Biochim Biophys Acta. 1991 Jan 17;1088(1):95-103. doi: 10.1016/0167-4781(91)90157-h.

DOI:10.1016/0167-4781(91)90157-h

PMID:1989698

Abstract

A full-length cDNA clone of the human pregnancy zone protein (PZP) was cloned from the hepatocellular carcinoma cell line Hep3B. Based on the exon sequences of the PZP gene (Devriendt et al. (1989) Gene 81, 325-334; Marynen et al., unpublished data), primer pairs were designed to amplify six overlapping fragments of the PZP cDNA. The obtained cDNA is 4609 bp long and contains an open reading frame coding for 1482 amino acids, including a signal peptide of 25 amino acid residues. Comparison with the published partial PZP amino acid sequence (Sottrup-Jensen et al. (1984) Proc. Natl. Acad. Sci. USA 81, 7353-7357) and the PZP genomic sequences confirmed the identity as a PZP cDNA. 71% of the corresponding amino acid residues in PZP and human alpha 2-macroglobulin (alpha 2M) are identical and all cysteine residues are conserved. A typical internal thiol ester site and a bait domain were identified. A Pro/Thr polymorphism was identified at amino acid position 1180, and an A/G nucleotide polymorphism at bp 4097.

摘要

从肝癌细胞系Hep3B中克隆出人类妊娠区蛋白（PZP）的全长cDNA克隆。根据PZP基因的外显子序列（Devriendt等人，（1989年）《基因》81卷，325 - 334页；Marynen等人，未发表数据），设计引物对以扩增PZP cDNA的六个重叠片段。获得的cDNA长4609 bp，包含一个编码1482个氨基酸的开放阅读框，其中包括一个25个氨基酸残基的信号肽。与已发表的部分PZP氨基酸序列（Sottrup - Jensen等人，（1984年）《美国国家科学院院刊》81卷，7353 - 7357页）和PZP基因组序列进行比较，证实其为PZP cDNA。PZP中71%的相应氨基酸残基与人类α2 - 巨球蛋白（α2M）相同，并且所有半胱氨酸残基均保守。鉴定出一个典型的内部硫酯位点和一个诱饵结构域。在氨基酸位置1180处鉴定出一个脯氨酸/苏氨酸多态性，在第4097 bp处鉴定出一个A/G核苷酸多态性。