卡介苗1619c基因对于牛分枝杆菌卡介苗在人THP-1和A549细胞系中的侵袭和细胞内存活并非必需。
The BCG1619c gene is not essential for invasion and intracellular persistence of Mycobacterium bovis BCG in human THP-1 and A549 cell lines.
作者信息
Florio Walter, Brancatisano Franca Lisa, Bottai Daria, Esin Semih, Di Luca Mariagrazia, Counoupas Claudio, Maisetta Giuseppantonio, Lupetti Antonella, Batoni Giovanna, Campa Mario
机构信息
Dipartimento di Patologia Sperimentale, Universita di Pisa, Italy.
出版信息
Can J Microbiol. 2009 Aug;55(8):975-82. doi: 10.1139/w09-053.
The BCG1619c gene of Mycobacterium bovis bacillus Calmette-Guérin (BCG) encodes for a 24 kDa invasin-like protein and is identical to the Rv1566c gene of Mycobacterium tuberculosis. To assess whether this protein was necessary for entry and (or) intracellular persistence in professional phagocytes and (or) in lung epithelial cells, a BCG1619c knockout mutant of M. bovis BCG was generated and compared with the parental BCG strain for its ability to infect and multiply in human monocyte derived THP-1 cells and in the lung epithelial cell line A549. No significant difference between the mutated and the parental BCG strain was observed in either of these in vitro infection systems, indicating that the BCG1619c gene is not essential for cell invasion and intracellular growth of BCG.
卡介苗（BCG）的牛分枝杆菌卡介苗BCG1619c基因编码一种24 kDa的侵袭素样蛋白，并且与结核分枝杆菌的Rv1566c基因相同。为了评估该蛋白对于专职吞噬细胞和（或）肺上皮细胞的侵入及（或）细胞内存活是否必要，构建了牛分枝杆菌卡介苗的BCG1619c基因敲除突变体，并将其与亲本卡介苗菌株在感染人单核细胞衍生的THP-1细胞及肺上皮细胞系A549并在其中增殖的能力方面进行比较。在这些体外感染系统中，未观察到突变的卡介苗菌株与亲本卡介苗菌株之间存在显著差异，这表明BCG1619c基因对于卡介苗的细胞侵袭和细胞内生长并非必需。
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