Bigi F, Gioffré A, Klepp L, Santangelo M P, Velicovsky C A, Giambartolomei G H, Fossati C A, Romano M I, Mendum T, McFadden J J, Cataldi A
Instituto de Biotecnología, CICV-INTA, De los Reseros y De las Cabañas, 1712, Castelar, Argentina.
Tuberculosis (Edinb). 2005 Jul;85(4):221-6. doi: 10.1016/j.tube.2004.11.002.
P36 is a member of a family of secreted proteins distributed throughout the genus Mycobacterium. The central domain of these proteins contains several amino acid PGLTS repeats, which differ considerably between species. P36, also called exported repetitive protein (Erp) in M. tuberculosis, has been shown to be associated with virulence since the disruption of its gene impaired multiplication of both virulent M. tuberculosis and M. bovis BCG in cultured macrophages and immunocompetent mice. In order to demonstrate that P36 is a putative virulence factor of wild-type Mycobacterium bovis we generated a P36 mutant by gene disruption and we evaluated its replication in spleen and lungs of infected mice. In this study, the mutant strain displays low levels of multiplication in mice, indicating that the P36 gene is important for in vivo growth of M. bovis.
P36是一类分泌蛋白家族的成员，分布于整个分枝杆菌属。这些蛋白的中央结构域包含几个氨基酸PGLTS重复序列，不同物种之间差异很大。P36在结核分枝杆菌中也被称为输出重复蛋白（Erp），自其基因被破坏会损害有毒力的结核分枝杆菌和卡介苗在培养的巨噬细胞及具有免疫活性的小鼠中的增殖以来，已显示它与毒力相关。为了证明P36是野生型牛分枝杆菌的一种假定毒力因子，我们通过基因破坏产生了一个P36突变体，并评估了其在感染小鼠脾脏和肺部中的复制情况。在本研究中，突变菌株在小鼠体内的增殖水平较低，表明P36基因对牛分枝杆菌的体内生长很重要。
