Kwinkowski Marek, Grabowski Sebastian, Konieczna Iwona, Nazarenko Evgeny L, Kaca Wiesław
Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland.
Pol J Microbiol. 2009;58(3):275-8.
The serological cross-reactivity between lipopolysaccharides (LPS) of S. fidelis KMM3582(T) and rabbit anti-O P. mirabilis antibodies was tested. Using ELISA and Western blot cross-reactivity between S. fidelis LPS and antisera against P. mirabilis O14, O3 LPSs was found. The observed cross-reaction may suggest that anti-P. mirabilis S1959 (O3) antibodies may bind to the internal part of S. fidelis O-polysaccharides. A weak interaction between S. fidelis LPS and antiserum against P. mirabilis O13 in Western blot suggests that the absolute configuration of non-sugar "AlaLys" component (N(epsilon)-[(S)-l-carboxyethyl]-N(alpha)-(D-galacturonoyl)-L-lysine) may influence the affinity of antibodies for S. fidelis LPS.
对忠诚链霉菌KMM3582(T)的脂多糖（LPS）与兔抗奇异变形杆菌O抗体之间的血清学交叉反应性进行了检测。采用酶联免疫吸附测定（ELISA）和蛋白质印迹法，发现忠诚链霉菌LPS与抗奇异变形杆菌O14、O3 LPS的抗血清之间存在交叉反应性。观察到的交叉反应可能表明，抗奇异变形杆菌S1959（O3）抗体可能与忠诚链霉菌O-多糖的内部部分结合。蛋白质印迹法中忠诚链霉菌LPS与抗奇异变形杆菌O13抗血清之间的弱相互作用表明，非糖“AlaLys”成分（N(ε)-[(S)-1-羧乙基]-N(α)-(D-半乳糖醛酰基)-L-赖氨酸）的绝对构型可能会影响抗体对忠诚链霉菌LPS的亲和力。
