Gleńska-Olender J, Durlik K, Konieczna I, Kowalska P, Gawęda J, Kaca W
Institute of Biology, Jan Kochanowski University, 25-406, Kielce, Poland.
Świętokrzyskie Biobank, The Regional Science and Technology Center, 26-060, Podzamcze, Poland.
Antonie Van Leeuwenhoek. 2017 Nov;110(11):1435-1443. doi: 10.1007/s10482-017-0937-0. Epub 2017 Sep 9.
Bacteria of the genus Proteus of the family Enterobacteriaceae are facultative human pathogens responsible mainly for urinary tract and wound infections, bacteremia and the development of rheumatoid arthritis (RA). We have analyzed and compared by ELISA the titer of antibodies in plasmas of healthy individuals and in sera of rheumatoid arthritis patients recognizing a potential host cross-reactive epitope (lysine-galacturonic acid epitopes) present in Proteus lipopolysaccharide (LPS). In our experiments LPSs isolated from two mutants of smooth Proteus mirabilis 1959 (O3), i.e. strains R110 and R45, were used. R110 (Ra type mutant) is lacking the O-specific polysaccharide, but possesses a complete core oligosaccharide, while R45 (Re type) has a reduced core oligosaccharide and contains two 3-deoxy-D-manno-oct-2-ulosonic acid residues and one of 4-amino-4-deoxy-L-arabinopyranose residues. Titer of P. mirabilis S1959 LPS-specific-antibodies increased with the age of blood donors. RA and blood donors' sera contained antibodies against S and Ra and Re type of P. mirabilis O3 LPSs. Antibodies recognizing lysine-galacturonic acid epitopes of O3 LPS were detected by ELISA in some plasmas of healthy individuals and sera of rheumatoid arthritis patients. RA patients antibodies reacting with P. mirabilis S1959 S and R LPSs may indicate a potential role of anti-LPS antibodies in molecular mimicry in RA diseases.
肠杆菌科变形杆菌属细菌是兼性人类病原体，主要导致尿路感染、伤口感染、菌血症以及类风湿性关节炎（RA）的发生。我们通过酶联免疫吸附测定（ELISA）分析并比较了健康个体血浆和类风湿性关节炎患者血清中识别变形杆菌脂多糖（LPS）中潜在宿主交叉反应表位（赖氨酸 - 半乳糖醛酸表位）的抗体滴度。在我们的实验中，使用了从光滑型奇异变形杆菌1959（O3）的两个突变体中分离出的LPS，即菌株R110和R45。R110（Ra型突变体）缺乏O特异性多糖，但拥有完整的核心寡糖，而R45（Re型）的核心寡糖减少，含有两个3 - 脱氧 - D - 甘露糖 - 辛 - 2 - 酮酸残基和一个4 - 氨基 - 4 - 脱氧 - L - 阿拉伯吡喃糖残基。奇异变形杆菌S1959 LPS特异性抗体的滴度随着献血者年龄的增长而增加。RA患者和献血者的血清中含有针对奇异变形杆菌O3 LPS的S型、Ra型和Re型的抗体。通过ELISA在一些健康个体的血浆和类风湿性关节炎患者的血清中检测到了识别O3 LPS赖氨酸 - 半乳糖醛酸表位的抗体。RA患者中与奇异变形杆菌S1959 S型和R型LPS反应的抗体可能表明抗LPS抗体在RA疾病分子模拟中具有潜在作用。
