School of Materials Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Langmuir. 2010 Mar 16;26(6):4320-6. doi: 10.1021/la903422q.
In this work, a novel avenue to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities to be used in the design of highly stable, magnetically separable enzyme-based systems was explored. As a model system, immobilization of acetylcholinesterase (AChE) was investigated using biomagnetic glasses composed of a magnetic core with a size tunable porous silica shell. The efficiency of the immobilization was determined by analyzing the biosensing capability of these biomagnetic glasses for the detection of the organophosphorous pesticide paraoxon. Screen printed electrodes with the AChE-biomagnetic glasses showed higher current response and stability than for the free enzyme. The detection limit of the paraoxon biosensor was in the nanomolar range.
在这项工作中，探索了一种新途径，以创建一种通用的方法来制造具有磁性功能的生物功能材料，用于设计高度稳定、可通过磁场分离的基于酶的系统。作为模型系统，研究了通过由具有可调节多孔硅壳的磁性核组成的生物磁玻璃固定乙酰胆碱酯酶（AChE）。通过分析这些生物磁玻璃对有机磷农药对氧磷检测的生物传感能力来确定固定化的效率。与游离酶相比，具有 AChE-生物磁玻璃的丝网印刷电极显示出更高的电流响应和稳定性。对氧磷生物传感器的检测限在纳摩尔范围内。
