Bender Joseph S, Kinyon Joann M, Kariyawasam Subhashinie, Halbur Patrick G, Opriessnig Tanja
Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
J Vet Diagn Invest. 2009 Nov;21(6):863-8. doi: 10.1177/104063870902100617.
The objective of the current study was to compare the diagnostic performance of a direct isolation method for Erysipelothrix spp. with a broth-based enrichment technique. Samples were obtained from three sources: 1) experimentally inoculated pigs, 2) porcine tissue samples submitted to the Iowa State University Veterinary Diagnostic Laboratory (Ames, IA), and 3) tissues from condemned carcasses at an abattoir. Culture plates from direct isolation and broth-based technique were evaluated for growth at 24 and 48 hr. Results indicated that the broth enrichment method was markedly more sensitive for the isolation of Erysipelothrix spp. To the authors' knowledge, this is the first comparison of direct culture and broth-based enrichment methods for the isolation of Erysipelothrix spp. Interestingly, in several samples, a Gram-positive bacterium with almost identical growth characteristics to Erysipelothrix spp. was detected and identified as a Vagococcus sp. through 16S ribosomal RNA gene sequencing. The results of this study indicate that the broth-based enrichment method should be used for the isolation of Erysipelothrix spp. from clinical samples with a history suggestive of erysipelas and that Vagococcus spp. is potentially an important differential diagnosis.
本研究的目的是比较红斑丹毒丝菌属直接分离方法与基于肉汤的富集技术的诊断性能。样本来自三个来源：1）实验接种的猪；2）提交给爱荷华州立大学兽医诊断实验室（艾姆斯，爱荷华州）的猪组织样本；3）屠宰场中被判定不合格的 carcasses 的组织。对直接分离和基于肉汤技术的培养平板在24小时和48小时时的生长情况进行评估。结果表明，肉汤富集法在分离红斑丹毒丝菌属方面明显更敏感。据作者所知，这是首次对直接培养和基于肉汤的富集方法分离红斑丹毒丝菌属进行比较。有趣的是，在几个样本中，检测到一种革兰氏阳性菌，其生长特征与红斑丹毒丝菌属几乎相同，并通过16S核糖体RNA基因测序鉴定为漫游球菌属。本研究结果表明，基于肉汤的富集方法应用于从有丹毒病史的临床样本中分离红斑丹毒丝菌属，且漫游球菌属可能是一个重要的鉴别诊断。
