Takahashi Eiji, Kuwayama Hideto, Kawamoto Keiko, Matsui Takane, Inokuma Hisashi
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan.
J Vet Diagn Invest. 2009 Nov;21(6):874-7. doi: 10.1177/104063870902100620.
Two-dimensional electrophoresis and immunoblotting were used to characterize 7 serum amyloid A (SAA) isoforms in cows with amyloidosis or chronic inflammation. Five SAA isoforms (isoelectric point [pI] 5.6, 6.1, 6.4, 6.8, and 7.7) were detected in all 10 amyloidosis serum samples, while 2 isoforms, pI 5.2 and 8.6, were detected in 4 and 9 of the samples, respectively. The same 7 isoforms were also detected in the serum of cows with chronic inflammation, but SAA pI 5.2 and 8.6 were detected in only 1 and 2 of 10 samples, respectively. It was concluded that although an amyloid-specific SAA isoform was not detected, examination of SAA isoform detection patterns may help identify bovine amyloidosis.
二维电泳和免疫印迹法用于鉴定患淀粉样变性或慢性炎症奶牛的7种血清淀粉样蛋白A（SAA）亚型。在所有10份淀粉样变性血清样本中均检测到5种SAA亚型（等电点[pI] 5.6、6.1、6.4、6.8和7.7），而在4份和9份样本中分别检测到2种亚型，pI 5.2和8.6。在患有慢性炎症的奶牛血清中也检测到相同的7种亚型，但在10份样本中，SAA pI 5.2和8.6仅分别在1份和2份样本中检测到。得出的结论是，尽管未检测到淀粉样特异性SAA亚型，但检查SAA亚型检测模式可能有助于识别牛淀粉样变性。
