牛血清淀粉样蛋白A亚型的检测

Detection of serum amyloid A isoforms in cattle.

作者信息

Takahashi Eiji, Kuwayama Hideto, Kawamoto Keiko, Matsui Takane, Inokuma Hisashi

机构信息

Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan.

出版信息

J Vet Diagn Invest. 2009 Nov;21(6):874-7. doi: 10.1177/104063870902100620.

DOI:10.1177/104063870902100620

PMID:19901294

Abstract

Two-dimensional electrophoresis and immunoblotting were used to characterize 7 serum amyloid A (SAA) isoforms in cows with amyloidosis or chronic inflammation. Five SAA isoforms (isoelectric point [pI] 5.6, 6.1, 6.4, 6.8, and 7.7) were detected in all 10 amyloidosis serum samples, while 2 isoforms, pI 5.2 and 8.6, were detected in 4 and 9 of the samples, respectively. The same 7 isoforms were also detected in the serum of cows with chronic inflammation, but SAA pI 5.2 and 8.6 were detected in only 1 and 2 of 10 samples, respectively. It was concluded that although an amyloid-specific SAA isoform was not detected, examination of SAA isoform detection patterns may help identify bovine amyloidosis.

摘要

二维电泳和免疫印迹法用于鉴定患淀粉样变性或慢性炎症奶牛的7种血清淀粉样蛋白A（SAA）亚型。在所有10份淀粉样变性血清样本中均检测到5种SAA亚型（等电点[pI] 5.6、6.1、6.4、6.8和7.7），而在4份和9份样本中分别检测到2种亚型，pI 5.2和8.6。在患有慢性炎症的奶牛血清中也检测到相同的7种亚型，但在10份样本中，SAA pI 5.2和8.6仅分别在1份和2份样本中检测到。得出的结论是，尽管未检测到淀粉样特异性SAA亚型，但检查SAA亚型检测模式可能有助于识别牛淀粉样变性。