UNL Center for Biotechnology and Plant Science Initiative, UNL, Lincoln, NE, USA.
Plant Signal Behav. 2009 Nov;4(11):1049-58. doi: 10.4161/psb.4.11.10103. Epub 2009 Nov 15.
Plants respond to environmental stresses by altering transcription of genes involved in the response. The chromatin modifier ATX1 regulates expression of a large number of genes; consequently, factors that affect ATX1 activity would also influence expression from ATX1-regulated genes. Here, we demonstrate that dehydration is such a factor implicating ATX1 in the plant's response to drought. In addition, we report that a hitherto unknown Arabidopsis gene, At3g10550, encodes a phosphoinositide 3'-phosphatase related to the animal myotubularins (AtMTM1). Myotubularin activities in plants have not been described and herein, we identify an overlapping set of genes co-regulated by ATX1 and AtMTM under drought conditions. We propose that these shared genes represent the ultimate targets of partially overlapping branches of the pathways of the nuclear ATX1 and the cytoplasmic AtMTM1. Our analyses offer first genome-wide insights into the relationship of an epigenetic factor and a lipid phosphatase from the other end of a shared drought responding pathway in Arabidopsis.
植物通过改变参与响应的基因的转录来应对环境胁迫。染色质修饰因子 ATX1 调节大量基因的表达;因此,影响 ATX1 活性的因素也会影响 ATX1 调节基因的表达。在这里,我们证明脱水是这样一个因素,将 ATX1 牵连到植物对干旱的反应中。此外,我们报告了一个迄今为止未知的拟南芥基因,At3g10550,编码一种与动物肌管素(AtMTM1)相关的磷酸肌醇 3′-磷酸酶。植物中的肌管素活性尚未被描述,在此,我们鉴定了一组在干旱条件下由 ATX1 和 AtMTM1 共同调控的重叠基因。我们提出,这些共享的基因代表了核 ATX1 和细胞质 AtMTM1 的部分重叠途径的最终靶标。我们的分析提供了第一个全基因组的见解,了解一个表观遗传因子和一个脂质磷酸酶在拟南芥共享的干旱响应途径的另一端的关系。