Cheung A L, Fischetti V A
Laboratory of Bacteriology and Immunology, Rockefeller University, New York, New York 10021.
J Surg Res. 1991 Feb;50(2):150-5. doi: 10.1016/0022-4804(91)90239-i.
The use of tampons and surgical gauze pads and colonization with Staphylococcus aureus have been established as risk factors for the development of toxic shock syndrome. To elucidate the role of blood factors in the mediation of staphylococcal adherence to fibers used in tampons and surgical packing, an adherence assay with cotton fibers was developed. Results demonstrated that cotton disks precoated with fibrinogen in the presence of human serum albumin bound a significant percentage of the inoculum for both staphylococcal strains tested when compared to human serum albumin controls. Likewise, fibers pretreated with plasma or defibrinated blood containing a small amount of fibrin revealed comparable staphylococcal adherence to that of fibrinogen. In contrast, fibers pretreated with serum, fibronectin, or vitronectin did not exhibit significant augmentation in staphylococcal attachment in comparison to human serum albumin controls. The attachment of staphylococci to fibrinogen and/or fibrin appeared to be specific and is blocked by goat anti-human fibrinogen antibody, but not fibronectin, vitronectin, or nonimmune goat IgG. Thus, our data indicate that fibrinogen/fibrin is the dominant blood component in the mediation of staphylococcal adherence to fibers used in tampons and surgical gauze pads.
使用卫生棉条和手术纱布垫以及金黄色葡萄球菌定植已被确认为中毒性休克综合征发生的危险因素。为了阐明血液因子在介导葡萄球菌黏附于卫生棉条和手术敷料所用纤维中的作用，开发了一种用棉纤维进行的黏附试验。结果表明，在人血清白蛋白存在的情况下预先包被纤维蛋白原的棉盘，与作为对照的人血清白蛋白相比，对所测试的两种葡萄球菌菌株的接种物均结合了相当大的百分比。同样，用含有少量纤维蛋白的血浆或去纤维蛋白血预处理的纤维显示出与纤维蛋白原相当的葡萄球菌黏附情况。相比之下，与作为对照的人血清白蛋白相比，用血清、纤连蛋白或玻连蛋白预处理的纤维在葡萄球菌附着方面未表现出显著增加。葡萄球菌对纤维蛋白原和/或纤维蛋白的附着似乎具有特异性，并且被山羊抗人纤维蛋白原抗体阻断，但不被纤连蛋白、玻连蛋白或非免疫山羊IgG阻断。因此，我们的数据表明，纤维蛋白原/纤维蛋白是介导葡萄球菌黏附于卫生棉条和手术纱布垫所用纤维的主要血液成分。
