Cheung A L, Fischetti V A
Laboratory of Bacteriology and Immunology, Rockefeller University, New York 10021.
J Clin Invest. 1989 Jun;83(6):2041-9. doi: 10.1172/JCI114115.
To study the role of surface proteins in the adherence of Staphylococcus aureus to fibers that are used in tampon and surgical gauze pad manufacture, we have developed an adherence assay with S. aureus cells and cotton and rayon fibers. Results suggest that staphylococcal adherence is dependent on both the substrate and the material used to coat these fibers. Scanning electron micrographs supported the adherence results and revealed more cells on the surface of cotton than rayon fibers. Treatment of staphylococcal cells with proteolytic enzymes significantly reduced binding to pure cotton and detergent-treated cotton fibers. Immunoblot analysis of cell wall proteins suggested that surface proteins in the mol wt range of 120-220 kD were involved in the adherence of S. aureus to cotton fibers. Although the adherence of S. aureus to cotton fibers alone appeared to be mediated through surface charge or hydrophobic interactions, bacterial binding to fibers which have been pretreated with defibrinated blood appeared to be more specific and independent of the surface constituents of the fibers. The results of these studies implicate staphylococcal surface proteins in the adherence of S. aureus to commercially available tampon fibers and surgical gauze pads.
为了研究表面蛋白在金黄色葡萄球菌黏附于用于制造卫生棉条和手术纱布垫的纤维过程中的作用，我们开发了一种金黄色葡萄球菌细胞与棉花和人造纤维的黏附试验。结果表明，葡萄球菌的黏附取决于底物以及用于包被这些纤维的材料。扫描电子显微镜照片支持了黏附结果，并显示棉花表面的细胞比人造纤维上的更多。用蛋白水解酶处理葡萄球菌细胞可显著降低其与纯棉和经洗涤剂处理的棉纤维的结合。对细胞壁蛋白的免疫印迹分析表明，分子量在120 - 220 kD范围内的表面蛋白参与了金黄色葡萄球菌对棉纤维的黏附。尽管金黄色葡萄球菌对纯棉纤维的黏附似乎是通过表面电荷或疏水相互作用介导的，但细菌与经去纤维蛋白血液预处理的纤维的结合似乎更具特异性，且与纤维的表面成分无关。这些研究结果表明，葡萄球菌表面蛋白参与了金黄色葡萄球菌对市售卫生棉条纤维和手术纱布垫的黏附。
