Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84108, USA.
J Appl Toxicol. 2010 Apr;30(3):212-7. doi: 10.1002/jat.1486.
Using a series of gold nanoparticles with incremental increase in dimensions but varying geometries (spherical vs rods) we have evaluated the influence of shape, size, surface properties and concentration on cellular uptake, adsorption of proteins and toxicity in a human prostate cancer cell line (PC-3). In the range of 30-90 nm diameter studied, spherical particles of 50 nm in diameter without polyethylene glycol (PEG) had the highest uptake. Surface attachment of PEG reduced cellular uptake. PEGylated gold nanorods had a net positive charge compared with their spherical counterparts and particle geometry influenced cellular uptake. In the absence of serum proteins the uptake of plain spherical GNPs increased. These studies pave the way for the tailoring of gold nanoparticles for targeted tumor therapy applications.
我们使用了一系列尺寸逐渐增加但形状不同的金纳米粒子（球形与棒形），评估了形状、尺寸、表面特性和浓度对人前列腺癌细胞系（PC-3）摄取、蛋白质吸附和毒性的影响。在研究的 30-90nm 直径范围内，直径为 50nm 且不带聚乙二醇（PEG）的球形粒子摄取率最高。PEG 接枝的金纳米棒与它们的球形对应物相比带有净正电荷，且粒子形状影响细胞摄取。在没有血清蛋白的情况下，普通球形 GNPs 的摄取增加。这些研究为针对肿瘤治疗应用的金纳米粒子的定制铺平了道路。
