Nishida Asuka, Sawada Yuka, Arai Rion, Ishibashi Naoki, Suzuo Miho, Ohno Akiko, Ashikaga Takao, Iijima Kazutoshi
Graduate School of Engineering Science, Yokohama National University, Yokohama, Japan.
College of Engineering Science, Yokohama National University, Yokohama, Japan.
Front Toxicol. 2024 Jul 1;6:1293147. doi: 10.3389/ftox.2024.1293147. eCollection 2024.
With the expansion of nanomaterials (NMs) usage, concerns about their toxicity are increasing, and the wide variety of NMs makes it difficult to assess their toxicity. Therefore, the development of a high-throughput, accurate, and certified method to evaluate the immunotoxicity of NMs is required. In this study, we assessed the immunotoxicity potential of various NMs, such as nanoparticles of silver, silica, and titanium dioxide, using the human Cell Line Activation Test (h-CLAT) at the cellular level. After exposure to silver nanoparticle dispersions, the expression levels of CD86 and CD54 increased, suggesting the activation of antigen-presenting cells (APCs) by silver nanoparticles. Quantification of silver ions eluted from silver nanoparticles and the activation of APCs by silver ions suggested that it was due to the release of silver ions. Silica nanoparticles also increased the expression of CD86 and/or CD54, and their activation ability correlated with the synthesis methods and hydrodynamic diameters. The ability of titanium dioxide to activate APCs differed depending on the crystal type and hydrodynamic diameter. These results suggest a potential method to evaluate the immunotoxicity potential of various NMs based on their ability to activate APCs using human monocytic THP-1 cells. This method will be valuable in assessing the immunotoxicity potential and elucidating the immunotoxic mechanisms of NMs.
随着纳米材料（NMs）使用的扩大，对其毒性的担忧日益增加，并且纳米材料种类繁多，难以评估其毒性。因此，需要开发一种高通量、准确且经过认证的方法来评估纳米材料的免疫毒性。在本研究中，我们在细胞水平上使用人细胞系激活试验（h-CLAT）评估了各种纳米材料的免疫毒性潜力，这些纳米材料包括银、二氧化硅和二氧化钛纳米颗粒。暴露于银纳米颗粒分散液后，CD86和CD54的表达水平升高，表明银纳米颗粒激活了抗原呈递细胞（APC）。对从银纳米颗粒中洗脱的银离子进行定量以及银离子对APC的激活表明，这是由于银离子的释放所致。二氧化硅纳米颗粒也增加了CD86和/或CD54的表达，并且它们的激活能力与合成方法和流体动力学直径相关。二氧化钛激活APC的能力因晶体类型和流体动力学直径而异。这些结果表明了一种基于纳米材料使用人单核细胞THP-1细胞激活APC的能力来评估其免疫毒性潜力的潜在方法。该方法在评估纳米材料的免疫毒性潜力和阐明其免疫毒性机制方面将具有重要价值。
