Bai Meng-Hai, Ge Bao-Feng, Wei Zhe, Bai Jie, Cheng Zi-Feng
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China.
Zhongguo Gu Shang. 2009 Oct;22(10):727-9.
To investigate the effect of pulsed electromagnetic fields (PEMs) on inducing osteoclastic like cell (OLC) formation changes and apoptosis in ovariectomized (OVX) rats bone marrow culture in vitro.
Thirty healthy three-month-old female Wistar rats were either sham-operated (Sham) or ovariectomized (OVX) and randomly divided into three groups: group A (OVX + PEMs, 18 rats), group B (OVX, 6 rats) and group C (Sham, 6 rats); group A was again randomly divided into three groups: A1, A2, A3. The frequencies adopted were 1.5, 2, 75 Hz and 30 minutes for once a day. All rats were fed with normal diet for 3 months, then the bone marrow of all rats were cultured, 2 days later, group A cells (including group A1, A2, A3) were collected and exposed to different frequencies PEMs for 2 weeks (30 min/day). In order to observe the changes of osteoclasts and count their numbers, cells were taken for Wright Giemsa staining, tartrate-resistance acid phosphatase (TRAP) staining and Hoechst 33258 staining.
TRAP staining results indicated the number of OLC in group C was the least, then was group A2, A3, A1, B. The number of OLC in group B was remarkably increased (P < 0.01; vs group C, A2). The number of OLC in group B was significantly increased (P < 0.05; vs group A1, A3). Hoechst 33258 staining results indicated the number of apoptosis of OLC in group C was more than other groups, which of group C, A2 was significantly increased (P < 0.05; vs group B).
PEMs had decreased the formation of OLC and increased the number of apoptosis of OLC in ovariectomized (OVX) rats bone marrow culture in vitro, the effects of 2 Hz was the best. PEMs would be a new way of osteoporosis therapy.
探讨脉冲电磁场（PEMs）对去卵巢（OVX）大鼠体外骨髓培养中破骨样细胞（OLC）形成变化及凋亡的影响。
30只健康3月龄雌性Wistar大鼠，分别行假手术（Sham）或去卵巢手术（OVX），随机分为3组：A组（OVX + PEMs，18只大鼠）、B组（OVX，6只大鼠）和C组（Sham，6只大鼠）；A组再随机分为3组：A1、A2、A3。采用的频率为1.5、2、75 Hz，每天1次，每次30分钟。所有大鼠正常饮食喂养3个月，然后采集所有大鼠的骨髓进行培养，2天后，收集A组细胞（包括A1、A2、A3组），并使其暴露于不同频率的PEMs下2周（每天30分钟）。为观察破骨细胞的变化并计数其数量，取细胞进行瑞氏吉姆萨染色、抗酒石酸酸性磷酸酶（TRAP）染色和Hoechst 33258染色。
TRAP染色结果显示，C组OLC数量最少，其次是A2、A3、A1、B组。B组OLC数量显著增加（P < 0.01；与C组、A2组相比）。B组OLC数量明显增加（P < 0.05；与A1组、A3组相比）。Hoechst 33258染色结果显示，C组OLC凋亡数量多于其他组，C组、A2组的凋亡数量显著增加（P < 0.05；与B组相比）。
PEMs可减少去卵巢（OVX）大鼠体外骨髓培养中OLC的形成，并增加OLC的凋亡数量，其中2 Hz的效果最佳。PEMs有望成为骨质疏松症治疗的新方法。
