活性氧水平降低与抑制细胞凋亡、硫氧还蛋白表达增加和牛白血病病毒前病毒载量增加有关。

Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads.

机构信息

Université de Liège (ULg), Gembloux Agro-Bio Tech, Molecular and Cellular Biology, Gembloux, Belgium.

出版信息

Retrovirology. 2009 Nov 10;6:102. doi: 10.1186/1742-4690-6-102.

BACKGROUND

Bovine Leukemia virus (BLV) is a deltaretrovirus that induces lymphoproliferation and leukemia in ruminants. In ex vivo cultures of B lymphocytes isolated from BLV-infected sheep show that spontaneous apoptosis is reduced. Here, we investigated the involvement of reactive oxygen species (ROS) in this process.

RESULTS

We demonstrate that (i) the levels of ROS and a major product of oxidative stress (8-OHdG) are reduced, while the thioredoxin antioxidant protein is highly expressed in BLV-infected B lymphocytes, (ii) induction of ROS by valproate (VPA) is pro-apoptotic, (iii) inversely, the scavenging of ROS with N-acetylcysteine inhibits apoptosis, and finally (iv) the levels of ROS inversely correlate with the proviral loads.

CONCLUSION

Together, these observations underline the importance of ROS in the mechanisms of inhibition of apoptosis linked to BLV infection.

背景

牛白血病病毒（BLV）是一种δ逆转录病毒，可诱导反刍动物的淋巴增生和白血病。在从 BLV 感染的绵羊中分离出的 B 淋巴细胞的体外培养中，自发凋亡减少。在这里，我们研究了活性氧（ROS）在这个过程中的参与。

结果

我们证明了（i）ROS 的水平和氧化应激的主要产物（8-OHdG）降低，而硫氧还蛋白抗氧化蛋白在 BLV 感染的 B 淋巴细胞中高度表达，（ii）丙戊酸钠（VPA）诱导的 ROS 是促凋亡的，（iii）相反，用 N-乙酰半胱氨酸清除 ROS 可抑制凋亡，最后（iv）ROS 的水平与前病毒载量呈负相关。