Florins Arnaud, Gillet Nicolas, Asquith Becca, Debacq Christophe, Jean Geneviève, Schwartz-Cornil Isabelle, Bonneau Michel, Burny Arsène, Reichert Michal, Kettmann Richard, Willems Luc
National Fund for Scientific Research, Molecular and Cellular Biology Laboratory, 13 avenue Maréchal Juin, 5030 Gembloux, Belgium.
J Virol. 2006 Dec;80(24):11998-2008. doi: 10.1128/JVI.01447-06. Epub 2006 Oct 11.
Lymphocyte homeostasis is determined by a critical balance between cell proliferation and death, an equilibrium which is deregulated in bovine leukemia virus (BLV)-infected sheep. We have previously shown that an excess of proliferation occurs in lymphoid tissues and that the peripheral blood population is prone to increased cell death. To further understand the mechanisms involved, we evaluated the physiological role of the spleen in this accelerated turnover. To this end, B lymphocytes were labeled in vivo using a fluorescent marker (carboxyfluorescein diacetate succinimidyl ester), and the cell kinetic parameters (proliferation and death rates) of animals before and after splenectomy were compared. We show that the enhanced cell death observed in BLV-infected sheep is abrogated after splenectomy, revealing a key role of the spleen in B-lymphocyte dynamics.
淋巴细胞稳态由细胞增殖与死亡之间的关键平衡决定，这种平衡在感染牛白血病病毒（BLV）的绵羊中失调。我们之前已经表明，在淋巴组织中会发生过度增殖，并且外周血中的细胞群体易于发生细胞死亡增加。为了进一步了解其中涉及的机制，我们评估了脾脏在这种加速更新中的生理作用。为此，使用荧光标记物（羧基荧光素二乙酸琥珀酰亚胺酯）在体内标记B淋巴细胞，并比较了脾切除前后动物的细胞动力学参数（增殖率和死亡率）。我们发现，脾切除后，感染BLV的绵羊中观察到的细胞死亡增强现象消失了，这揭示了脾脏在B淋巴细胞动态变化中的关键作用。
