Cerqueira Daniella Ferraz, Portela Maristela Barbosa, Soares Rosangela Maria De Araujo, De Souza Ivete Pomarico Ribeiro, Castro Gloria Fernanda
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Gen Dent. 2009 Jul-Aug;57(4):438-41.
This study sought to compare techniques used to make a quantified evaluation of Candida spp. in children infected with HIV. Twenty-four HIV-infected children (age 3 to 13) were selected. Three sterilized swabs were used for each child: one for the dorsum of the tongue, one for the hard palate mucosa, and one for the right jugal mucosa; each swab was rubbed for 10 seconds and transferred to sterilized test tubes containing 1 mL of 0.9% saline solution. Candida spp. growth was observed in 95.8% of all samples, including 95.7% of tongue samples (Group T), 87.0% of saliva samples, 56.6% of hard palate mucosa samples (Group P), and 47.8% of right jugal mucosa samples (Group J). There was no statistical difference in Candida spp. growth between saliva samples and Group T samples, although both had higher growth compared to Groups P and J (p < 0.05; chi(2)). Regarding the sensitivity of each site for positive Candida spp. growth, Group T samples showed 69.5%, while saliva samples showed 52.2%, Group P samples showed 21.7%, and Group J samples showed 13.04%, with no significant statistical difference between Group T and saliva; however, both were more sensitive than Groups J and P (p < 0.05, chi(2)). It was concluded that whole stimulated saliva and swabbing the tongue were considered satisfactory for measuring Candida spp. in HIV-infected children.
本研究旨在比较用于对感染HIV的儿童念珠菌属进行定量评估的技术。选取了24名感染HIV的儿童（年龄3至13岁）。为每名儿童使用三根消毒棉签：一根用于舌背，一根用于硬腭黏膜，一根用于右侧颊黏膜；每根棉签擦拭10秒，然后转移至装有1 mL 0.9%盐溶液的消毒试管中。在所有样本中，95.8%观察到念珠菌属生长，其中舌部样本（T组）为95.7%，唾液样本为87.0%，硬腭黏膜样本（P组）为56.6%，右侧颊黏膜样本（J组）为47.8%。唾液样本和T组样本中念珠菌属生长无统计学差异，不过二者的生长率均高于P组和J组（p<0.05；卡方检验）。关于每个部位念珠菌属生长阳性的敏感性，T组样本为69.5%，唾液样本为52.2%，P组样本为21.7%，J组样本为13.04%，T组和唾液样本之间无显著统计学差异；然而，二者均比J组和P组更敏感（p<0.05，卡方检验）。得出的结论是，对于测量感染HIV儿童的念珠菌属，全刺激唾液和擦拭舌头被认为是令人满意的。
