Department of Bioengineering, University of Utah, 72 South Central Campus Dr., Salt Lake City, UT 84112, USA.
Lab Chip. 2009 Dec 7;9(23):3370-7. doi: 10.1039/b912881f. Epub 2009 Sep 18.
Single-cell electric impedance topography (sceTopo), a technique introduced here, maps the spatial distribution of capacitance (i.e. displacement current) associated with the membranes of isolated, living cells. Cells were positioned in the center of a circular recording chamber surrounded by eight electrodes. Electrodes were evenly distributed on the periphery of the recording chamber. Electric impedance measured between adjacent electrode pairs (10 kHz-5 MHz) was used to construct topographical maps of the spatial distribution of membrane capacitance. Xenopus Oocytes were used as a model cell to develop sceTopo because these cells consist of two visually distinguishable hemispheres, each with distinct membrane composition and structure. Results showed significant differences in the imaginary component of the impedance between the two oocyte hemispheres. In addition, the same circumferential array was used to map the size of the extracellular electrical shunt path around the cell, providing a means to estimate the location and shape of the cell in the recording chamber.
单细胞电阻抗形貌（sceTopo）是一种在此引入的技术，它绘制了与分离的活细胞的膜相关的电容（即位移电流）的空间分布。细胞被放置在由八个电极环绕的圆形记录室的中心。电极均匀分布在记录室的外围。通过测量相邻电极对之间的电阻抗（10 kHz-5 MHz）来构建膜电容空间分布的地形图谱。非洲爪蟾卵母细胞被用作sceTopo 的模型细胞，因为这些细胞由两个视觉上可区分的半球组成，每个半球都具有不同的膜组成和结构。结果表明，两个卵母细胞半球之间的阻抗虚部存在显著差异。此外，相同的圆周阵列被用于绘制细胞周围的细胞外电分流路径的大小，为估计记录室内细胞的位置和形状提供了一种手段。
