School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
Biosensors (Basel). 2022 Dec 27;13(1):30. doi: 10.3390/bios13010030.
Surface-enhanced Raman scattering (SERS) is a kind of popular non-destructive and water-free interference analytical technology with fast response, excellent sensitivity and specificity to trace biotargets in biological samples. Recently, many researches have focused on the preparation of various magnetic nanoparticle-based SERS substrates for developing efficient bioanalytical methods, which greatly improved the selectivity and accuracy of the proposed SERS bioassays. There has been a rapid increase in the number of reports about magnetic SERS substrates in the past decade, and the number of related papers and citations have exceeded 500 and 2000, respectively. Moreover, most of the papers published since 2009 have been dedicated to analytical applications. In the paper, the recent advances in magnetic nanoparticle-based SERS substrates for bioanalysis were reviewed in detail based on their various morphologies, such as magnetic core-shell nanoparticles, magnetic core-satellite nanoparticles and non-spherical magnetic nanoparticles and their different functions, such as separation and enrichment, recognition and SERS tags. Moreover, the typical application progress on magnetic nanoparticle-based SERS substrates for bioanalysis of amino acids and protein, DNA and RNA sequences, cancer cells and related tumor biomarkers, etc., was summarized and introduced. Finally, the future trends and prospective for SERS bioanalysis by magnetic nanoparticle-based substrates were proposed based on the systematical study of typical and latest references. It is expected that this review would provide useful information and clues for the researchers with interest in SERS bioanalysis.
表面增强拉曼散射（SERS）是一种流行的非破坏性和无水干扰分析技术，具有快速响应、对生物样品中痕量生物靶标的优异灵敏度和特异性。最近，许多研究都集中在制备各种基于磁性纳米粒子的 SERS 基底上，以开发高效的生物分析方法，这极大地提高了所提出的 SERS 生物分析的选择性和准确性。在过去的十年中，关于磁性 SERS 基底的报道数量迅速增加，相关论文和引文的数量分别超过 500 和 2000。此外，自 2009 年以来发表的大多数论文都致力于分析应用。本文详细综述了基于不同形态的磁性纳米粒子的 SERS 基底在生物分析中的最新进展，如磁性核壳纳米粒子、磁性核卫星纳米粒子和非球形磁性纳米粒子，以及它们不同的功能，如分离和富集、识别和 SERS 标签。此外，还总结和介绍了基于磁性纳米粒子的 SERS 基底在生物分析中用于氨基酸和蛋白质、DNA 和 RNA 序列、癌细胞和相关肿瘤生物标志物等方面的典型应用进展。最后，根据对典型和最新参考文献的系统研究，提出了基于磁性纳米粒子的 SERS 生物分析的未来趋势和展望。希望本文能为对 SERS 生物分析感兴趣的研究人员提供有用的信息和线索。
