Institute of Orthopedics, General Hospital of PLA, Beijing 100853, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2010 Feb;92(2):377-85. doi: 10.1002/jbm.b.31525.
Calcium phosphate cement (CPC) that can be injected to form a scaffold in situ has promise for the repair of bone defects. However, its low-strength limits the CPC to non-stress-bearing repairs. Fibrin glue (FG) with good sticking property and biocompatibility is possible used to reinforce the CPC. The objective of this study was to investigate the effects of FG on the mechanical and biological properties of CPC in an injectable CPC-FG composite. The initial setting time of this CPC-FG was delayed compared with the CPC control at different powder/liquid (P/L) mass ratio (p > 0.05). At a P/L of 5, the strength was (38.41 +/- 4.32) MPa for the CPC-FG, much higher than (27.42 +/- 2.85) MPa for the CPC alone (p < 0.05). SEM showed bone marrow stromal cells (BMSCs) with healthy spreading and anchored on the CPC-FG composite. After 14 days, the alkaline phosphatase (ALP) activity was (538 +/- 33) for the BMSCs on the CPC-FG and (517 +/- 27) for the BMSCs on the CPC alone. Both ALPs were higher than the baseline ALP (93 +/- 10) for the undifferentiated BMSCs (p < 0.05). The results demonstrate that this stronger CPC-FG scaffold may be useful for stem cell-based bone regeneration in moderate load-bearing orthopedic applications.
可注射形成原位支架的磷酸钙水泥（CPC）有望修复骨缺损。然而，其强度低限制了 CPC 用于非承重修复。具有良好黏附性和生物相容性的纤维蛋白胶（FG）可用于增强 CPC。本研究旨在探讨 FG 对可注射 CPC-FG 复合材料中 CPC 的机械和生物学性能的影响。不同粉末/液体（P/L）质量比（p > 0.05）下，CPC-FG 的初凝时间与 CPC 对照相比延迟。在 P/L 为 5 时，CPC-FG 的强度为（38.41 +/- 4.32）MPa，明显高于单独的 CPC 的强度（27.42 +/- 2.85）MPa（p < 0.05）。SEM 显示骨髓基质细胞（BMSCs）在 CPC-FG 复合材料上健康扩展和附着。14 天后，CPC-FG 上的 BMSCs 的碱性磷酸酶（ALP）活性为（538 +/- 33），而单独的 CPC 上的 BMSCs 的 ALP 活性为（517 +/- 27）。这两种 ALP 均高于未分化的 BMSCs 的基础 ALP（93 +/- 10）（p < 0.05）。结果表明，这种强度更高的 CPC-FG 支架可能有助于中等承重骨科应用中的基于干细胞的骨再生。
