Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, Fetscherstr 74, 01307 Dresden, Germany.
Department of Oral and Maxillofacial Surgery, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, Fetscherstr 74, 01307 Dresden, Germany.
Int J Mol Sci. 2021 Jan 26;22(3):1218. doi: 10.3390/ijms22031218.
Cleft alveolar bone defects can be treated potentially with tissue engineered bone grafts. Herein, we developed novel biphasic bone constructs consisting of two clinically certified materials, a calcium phosphate cement (CPC) and a fibrin gel that were biofabricated using 3D plotting. The fibrin gel was loaded with mesenchymal stromal cells (MSC) derived from bone marrow. Firstly, the degradation of fibrin as well as the behavior of cells in the biphasic system were evaluated in vitro. Fibrin degraded quickly in presence of MSC. Our results showed that the plotted CPC structure acted slightly stabilizing for the fibrin gel. However, with passing time and fibrin degradation, MSC migrated to the CPC surface. Thus, the fibrin gel could be identified as cell delivery system. A pilot study in vivo was conducted in artificial craniofacial defects in Lewis rats. Ongoing bone formation could be evidenced over 12 weeks but the biphasic constructs were not completely osseous integrated. Nevertheless, our results show that the combination of 3D plotted CPC constructs and fibrin as suitable cell delivery system enables the fabrication of novel regenerative implants for the treatment of alveolar bone defects.
裂牙槽骨缺损可以通过组织工程骨移植进行潜在治疗。在此，我们开发了由两种临床认可的材料组成的新型双相骨构建体，即磷酸钙水泥（CPC）和纤维蛋白凝胶，它们使用 3D 绘图进行生物制造。纤维蛋白凝胶负载有源自骨髓的间充质基质细胞（MSC）。首先，在体外评估了纤维蛋白的降解以及双相系统中细胞的行为。在 MSC 的存在下，纤维蛋白迅速降解。我们的结果表明，绘制的 CPC 结构对纤维蛋白凝胶具有轻微的稳定作用。然而，随着时间的推移和纤维蛋白的降解，MSC 迁移到 CPC 表面。因此，纤维蛋白凝胶可以被鉴定为细胞输送系统。在刘易斯大鼠的人工颅面缺损中进行了初步的体内研究。在 12 周内可以证明持续的骨形成，但双相构建体没有完全骨整合。然而，我们的结果表明，3D 绘制的 CPC 构建体与纤维蛋白的结合作为合适的细胞输送系统，能够制造用于治疗牙槽骨缺损的新型再生植入物。
