He Fang, Shen Qin, Jiang Hui, Zhou Jian, Cheng Jian, Guo Dadong, Li Qingning, Wang Xuemei, Fu Degang, Chen Baoan
State Key Laboratory of Bioelectronics, Chien-Shiung Wu Laboratory, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Talanta. 2009 Jan 15;77(3):1009-14. doi: 10.1016/j.talanta.2008.07.063. Epub 2008 Aug 14.
In this study, we have proposed a novel strategy for the rapid identification and high sensitive detection of different kinds of cancer cells by means of electrochemical and contact angle measurements. A simple, unlabeled method based on the functionalized Au nanoparticles (GNPs) modified interface has been utilized to distinguish the different cancer cells, including lung cancer cells, liver cancer cells, drug sensitive leukemia K562/B.W cells and drug resistant leukemia K562/ADM cells. The relevant results indicate that under optimal conditions, this method can provide the quantitative determination of cancer cells, with a detection limit of approximately 10(3)cells mL(-1). Our observations demonstrate that the difference in the hydrophilic properties for target cellular surfaces and in the uptake efficiency of the anticancer drug daunorubicin for different cancer cells could be readily chosen as the elements of cancer identification and sensitive detection. This raises the possibility to advance the promising clinic diagnosis and monitoring of tumors with the aim of successful chemotherapy of human cancers.
在本研究中，我们提出了一种通过电化学和接触角测量快速鉴定和高灵敏度检测不同种类癌细胞的新策略。基于功能化金纳米颗粒（GNPs）修饰界面的一种简单、无标记方法已被用于区分不同的癌细胞，包括肺癌细胞、肝癌细胞、药物敏感白血病K562/B.W细胞和耐药白血病K562/ADM细胞。相关结果表明，在最佳条件下，该方法能够对癌细胞进行定量测定，检测限约为10³个细胞·mL⁻¹。我们的观察结果表明，靶细胞表面亲水性的差异以及不同癌细胞对抗癌药物柔红霉素摄取效率的差异可很容易地被选作癌症鉴定和灵敏检测的要素。这增加了推进有望实现人类癌症成功化疗的肿瘤临床诊断和监测的可能性。
