Australian Centre for Plant Functional Genomics and School of Land Crop and Food Sciences, University of Queensland, Brisbane, Australia.
Plant Biotechnol J. 2010 Jan;8(1):2-9. doi: 10.1111/j.1467-7652.2009.00459.x. Epub 2009 Nov 9.
DNA sequencing technology is undergoing a revolution with the commercialization of second generation technologies capable of sequencing thousands of millions of nucleotide bases in each run. The data explosion resulting from this technology is likely to continue to increase with the further development of second generation sequencing and the introduction of third generation single-molecule sequencing methods over the coming years. The question is no longer whether we can sequence crop genomes which are often large and complex, but how soon can we sequence them? Even cereal genomes such as wheat and barley which were once considered intractable are coming under the spotlight of the new sequencing technologies and an array of new projects and approaches are being established. The increasing availability of DNA sequence information enables the discovery of genes and molecular markers associated with diverse agronomic traits creating new opportunities for crop improvement. However, the challenge remains to convert this mass of data into knowledge that can be applied in crop breeding programs.
DNA 测序技术正在发生一场革命，随着第二代技术的商业化，这些技术每次运行都能够对数十亿个核苷酸进行测序。随着第二代测序技术的进一步发展以及未来几年第三代单分子测序方法的引入，这种技术带来的数据爆炸很可能会继续增加。问题不再是我们是否能够对通常较大且复杂的作物基因组进行测序，而是我们能够多快对它们进行测序。即使是曾经被认为难以处理的谷物基因组，如小麦和大麦，也正在成为新测序技术的焦点，一系列新的项目和方法正在建立。越来越多的 DNA 序列信息的可用性使得发现与各种农艺性状相关的基因和分子标记成为可能，为作物改良创造了新的机会。然而，如何将这些海量数据转化为可应用于作物育种计划的知识仍然是一个挑战。
