Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy.
Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):3-10. doi: 10.1016/j.cimid.2009.09.004. Epub 2009 Nov 10.
Four DNA vaccines against BoHV-1 were evaluated for their efficacy in calves. Twelve animals were divided into four groups which were injected with four different DNA vaccines: pVAX-tgD (Vaccine A); pVAX-tgD co-immunised with pVAX-48CpG (Vaccine B); pVAX-UbiLacI-tgD-L (Vaccine C); pVAX-UbiLacI-tgD-L co-immunised with pVAX-48CpG (Vaccine D). Three additional calves were given the plasmid vector and served as controls. Ninety days after the first vaccination all calves were challenge infected with BoHV-1. All animals developed a severe form of infections bovine rhinotracheitis. Only the calves given the pVAX-tgD co-immunised with pVAX-48CpG (Vaccine B) developed humoral antibodies against BoHV-1 between 56 and 90 days after the first vaccination, whereas in calves of other groups and in the controls, antibodies appeared only after the infection. In the calves vaccinated with either pVAX-tgD (Vaccine A) or pVAX-tgD combined with pVAX-48CpG (Vaccine B), BoHV-1-specific IFN-γ secreting cells were detected in PBMCs 90 days after the first vaccination and their number increased after challenge exposure. In the other groups the IFN-γ secreting cells were detected after virus infection and at low values.
四种 BoHV-1 的 DNA 疫苗在犊牛中的功效进行了评估。12 只动物被分为四组，分别注射四种不同的 DNA 疫苗：pVAX-tgD（疫苗 A）；pVAX-tgD 与 pVAX-48CpG 共免疫（疫苗 B）；pVAX-UbiLacI-tgD-L（疫苗 C）；pVAX-UbiLacI-tgD-L 与 pVAX-48CpG 共免疫（疫苗 D）。另外 3 只牛接受了质粒载体作为对照。第一次接种后 90 天，所有犊牛均用 BoHV-1 攻毒。所有动物均发展为严重的牛传染性鼻气管炎。只有接种 pVAX-tgD 与 pVAX-48CpG 共免疫的牛（疫苗 B）在第一次接种后 56 至 90 天之间产生了针对 BoHV-1 的体液抗体，而在其他组和对照组的牛中，仅在感染后才出现抗体。在接种 pVAX-tgD（疫苗 A）或 pVAX-tgD 与 pVAX-48CpG（疫苗 B）联合接种的牛中，在第一次接种后 90 天可在 PBMC 中检测到 BoHV-1 特异性 IFN-γ 分泌细胞，并且在暴露于攻毒后其数量增加。在其他组中，在病毒感染后和低值时才检测到 IFN-γ 分泌细胞。
