Birt Diane F, Widrlechner Mark P, Hammer Kimberly Dp, Hillwig Matthew L, Wei Jingqiang, Kraus George A, Murphy Patricia A, McCoy Joeann, Wurtele Eve S, Neighbors Jeffrey D, Wiemer David F, Maury Wendy J, Price Jason P
The Center for Research on Botanical Dietary Supplements, Iowa State University, Ames, IA.
Pharm Biol. 2009;47(8):774-782. doi: 10.1080/13880200902988645.
The Iowa Center for Research on Botanical Dietary Supplements seeks to optimize Echinacea, Hypericum, and Prunella botanical supplements for human-health benefit, emphasizing antiviral, anti-inflammatory and anti-pain activities. This mini-review reports on ongoing studies on Hypericum. The Center uses the genetically diverse, well-documented Hypericum populations collected and maintained at the USDA-ARS North Central Regional Plant Introduction Station (NCRPIS), and the strength of research in synthetic chemistry at Iowa State University to tap natural diversity, to help discover key constituents and interactions among constituents that impact bioactivity and toxicity. The NCRPIS has acquired more than 180 distinct populations of Hypericum, with a focus on Hypericum perforatum L. (Hypericaceae), representing about 13% of currently recognized taxa. Center chemists have developed novel synthetic pathways for key flavones, acyl phloroglucinols, hyperolactones and a tetralin that have been found in Hypericum, and these compounds are used as standards and for bioactivity studies. Both light-dependent and light-independent anti-viral activities have been identified by using bioactivity-guided fractionation of H. perforatum and a HIV-1 infection test system. Our Center has focused on light-independent activity, potentially due to novel chemicals, and polar fractions are undergoing further fractionation. Anti-inflammatory activity has been found to be light-independent, and fractionation of a flavonoid-rich extract revealed four compounds (amentoflavone, chlorogenic acid, pseudohypericin and quercetin) that interacted in the light to inhibit lipopolysaccharide-induced prostaglandin E(2) activity. The Center continues to explore novel populations of H. perforatum and related species to identify constituents and interactions of constituents that contribute to potential health benefits related to infection.
爱荷华州植物性膳食补充剂研究中心致力于优化紫锥菊、金丝桃和夏枯草等植物性补充剂，以促进人类健康，重点关注抗病毒、抗炎和止痛活性。本综述报告了关于金丝桃的正在进行的研究。该中心利用在美国农业部农业研究局北中部地区植物引种站（NCRPIS）收集和保存的遗传多样、记录完善的金丝桃种群，以及爱荷华州立大学合成化学研究的优势，挖掘自然多样性，以帮助发现影响生物活性和毒性的关键成分及其相互作用。NCRPIS已获取了180多个不同的金丝桃种群，重点是贯叶连翘（金丝桃科），约占目前已确认分类群的13%。该中心的化学家已开发出金丝桃中发现的关键黄酮类化合物、酰基间苯三酚、金丝桃内酯和一种四氢萘的新型合成途径，这些化合物用作标准品并用于生物活性研究。通过对贯叶连翘进行生物活性导向分级分离和HIV-1感染测试系统，已确定了光依赖性和非光依赖性抗病毒活性。我们中心专注于非光依赖性活性，可能是由于新型化学物质，极性馏分正在进一步分级分离。已发现抗炎活性是非光依赖性的，对富含黄酮类化合物的提取物进行分级分离后发现了四种化合物（穗花杉双黄酮、绿原酸、假金丝桃素和槲皮素），它们在光照下相互作用以抑制脂多糖诱导的前列腺素E2活性。该中心继续探索贯叶连翘和相关物种的新种群，以确定有助于与感染相关潜在健康益处的成分及其相互作用。
