Department of Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11841, Egypt.
The Regional Center for Mycology and Biotechnology, Al-Azhar University, 11787 Nasr City, Cairo, Egypt.
Biomed Res Int. 2023 Apr 14;2023:6934398. doi: 10.1155/2023/6934398. eCollection 2023.
The perennial plant is widely distributed around the world. It has been used for many years in conventional medicine to treat a variety of illnesses, including stress, mild to moderate depression, and minor injuries. This study examined the antimicrobial activity of the total extract and its fractions (n-hexane, ethyl acetate, chloroform, and aqueous) against multi-drug-resistant (MDR) isolates that were gathered from clinical samples, including methicillin-resistant (MRSA), , , and .
Aerial parts of were collected and extracted using various solvents and were tested versus different isolated bacterial species. The inhibition zone of tested extracts was detected using an agar diffusion assay, and MICs were measured. Phytochemical analysis of promising extract was done using LC-ESI-MS/MS. Ultrastructure examination for the most altered bacteria used transmission electron microscopy. Antioxidant assays were done using DPPH and ABTS scavenging capacity methods. Cytotoxicity was reported versus Vero cells.
Different extracts of showed promising antibacterial activity against the pathogens. While the subfractions of the total extract were observed to show lesser inhibition zones and higher MIC values than the total extract of against MDR strains, the total extract of demonstrated the most potent antimicrobial action with an inhibition zone range of 17.9-27.9 mm. MDR- was discovered to be the most susceptible strain, which is consistent with the antibacterial inhibitory action of whole extract. Additionally, after treatment at the minimum inhibitory concentration (MIC 3.9 g/ml), the transmission electron microscope showed alterations in the ultrastructure of the cells. Methanol extract from has a CC value of 976.75 g/ml.
Future inhibitors that target MDR strains may be revealed by these findings. Additionally, the extracts that were put to the test demonstrated strong antioxidant effects as shown by DPPH or ABTS radical-scavenging assays.
多年生植物分布广泛，在传统医学中已使用多年，用于治疗多种疾病，包括压力、轻度至中度抑郁和轻伤。本研究检查了全提取物及其馏分（正己烷、乙酸乙酯、氯仿和水）对从临床样本中收集的多药耐药（MDR）分离株的抗菌活性，包括耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌、铜绿假单胞菌和鲍曼不动杆菌。
采集植物的地上部分，用各种溶剂提取，并对不同的分离细菌进行测试。使用琼脂扩散法检测测试提取物的抑菌圈，并测量 MIC 值。使用 LC-ESI-MS/MS 对有前途的提取物进行植物化学分析。使用透射电子显微镜对最受影响的细菌进行超微结构检查。使用 DPPH 和 ABTS 清除能力法进行抗氧化测定。报告了对 Vero 细胞的细胞毒性。
不同的提取物对病原体表现出有希望的抗菌活性。虽然总提取物的亚馏分显示出比总提取物对 MDR 菌株的抑制圈更小和更高的 MIC 值，但总提取物对 MDR 菌株表现出最强的抗菌作用，抑制圈范围为 17.9-27.9mm。发现 MDR-是最敏感的菌株，这与全提取物的抑菌作用一致。此外，在最低抑菌浓度（MIC 3.9g/ml）下处理后，透射电子显微镜显示 细胞的超微结构发生改变。来自 的甲醇提取物的 CC 值为 976.75g/ml。
这些发现可能揭示出针对 MDR 菌株的未来抑制剂。此外，经过测试的提取物在 DPPH 或 ABTS 自由基清除测定中表现出强烈的抗氧化作用。
