在存在血浆γ-球蛋白组分蛋白的情况下产生的干扰素-α的检测。

Detection of IFN-alpha produced in the presence of plasma gamma-globulin fraction proteins.

作者信息

Cheknyov S B, Babayants A A, Efremova I E, Yushkovets E N

机构信息

Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2009 May;147(5):613-6. doi: 10.1007/s10517-009-0569-0.

DOI:10.1007/s10517-009-0569-0

PMID:19907752

Abstract

Interferon-alpha was detected in IFN pool produced by human leukocytes in the presence of gamma-globulin fraction proteins, copper and zinc cations, and metal-modified gamma-globulins. The cytokine appeared in culture medium at early terms (24 h) of incubation, is characterized by acid resistance, and is neutralized by antibodies to IFN-alpha. The content of IFN-alpha in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum gamma-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation.

摘要

在存在γ-球蛋白级分蛋白、铜和锌阳离子以及金属修饰的γ-球蛋白的情况下，在人白细胞产生的干扰素池中检测到α-干扰素。这种细胞因子在培养早期（24小时）出现在培养基中，具有耐酸性，并且可被α-干扰素抗体中和。诱导白细胞上清液中α-干扰素的含量达到60 - 90 pg/ml，并且与样品的抗病毒活性相关。与人类血清γ-球蛋白结合的锌在培养早期减弱了该蛋白的干扰素诱导特性，而铜则刺激了该特性的实现。