用NotI连接克隆使多囊肾病基因区域饱和。
Saturating the region of the polycystic kidney disease gene with NotI linking clones.
作者信息
Himmelbauer H, Germino G G, Ceccherini I, Romeo G, Reeders S T, Frischauf A M
机构信息
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
出版信息
Am J Hum Genet. 1991 Feb;48(2):325-34.
Abstract
A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.
摘要
利用含人类16号染色体片段的辐射杂种构建了一个NotI连接文库。这些克隆在一组体细胞杂种上进行了定位，并且10个含NotI位点的不同克隆被定位在多囊肾病1（PKD1）基因座两侧遗传标记的附近及之间。通过脉冲场凝胶分析表明，这些克隆分布在覆盖1200 kb的四个相邻ClaI片段上。
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