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用NotI连接克隆使多囊肾病基因区域饱和。

Saturating the region of the polycystic kidney disease gene with NotI linking clones.

作者信息

Himmelbauer H, Germino G G, Ceccherini I, Romeo G, Reeders S T, Frischauf A M

机构信息

Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.

出版信息

Am J Hum Genet. 1991 Feb;48(2):325-34.

PMID:1990840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1683004/
Abstract

A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

摘要

利用含人类16号染色体片段的辐射杂种构建了一个NotI连接文库。这些克隆在一组体细胞杂种上进行了定位，并且10个含NotI位点的不同克隆被定位在多囊肾病1（PKD1）基因座两侧遗传标记的附近及之间。通过脉冲场凝胶分析表明，这些克隆分布在覆盖1200 kb的四个相邻ClaI片段上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca5/1683004/64918ddd199e/ajhg00086-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca5/1683004/071e92cf0a6b/ajhg00086-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca5/1683004/64918ddd199e/ajhg00086-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca5/1683004/071e92cf0a6b/ajhg00086-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca5/1683004/64918ddd199e/ajhg00086-0158-a.jpg

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1
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Am J Hum Genet. 1991 Feb;48(2):325-34.
2
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引用本文的文献

1
EagI and NotI linking clones from human chromosomes 11 and Xp.来自人类11号染色体和Xp染色体的EagI和NotI连接克隆。
Hum Genet. 1996 Jun;97(6):742-9. doi: 10.1007/BF02346183.
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Rubinstein-Taybi syndrome caused by submicroscopic deletions within 16p13.3.由16p13.3区域内亚显微缺失引起的鲁宾斯坦-泰比综合征。
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Construction of a map of chromosome 16 by using radiation hybrids.利用辐射杂种构建16号染色体图谱。

本文引用的文献

1
Cystic kidneys. Genetics, pathologic anatomy, clinical picture, and prenatal diagnosis.多囊肾。遗传学、病理解剖学、临床表现及产前诊断。
Hum Genet. 1984;68(2):104-35. doi: 10.1007/BF00279301.
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Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):104-8. doi: 10.1073/pnas.89.1.104.
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Long-range walking techniques in positional cloning strategies.定位克隆策略中的远程步行技术
Mamm Genome. 1992;3(3):127-42. doi: 10.1007/BF00352457.
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Molecular analysis of human chromosome 16 cosmid clones containing NotI sites.对含NotI位点的人类16号染色体黏粒克隆进行分子分析。
Mamm Genome. 1992;3(2):92-100. doi: 10.1007/BF00431252.
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Proc Natl Acad Sci U S A. 1981 Nov;78(11):7015-8. doi: 10.1073/pnas.78.11.7015.
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Isolation of genomic DNA.基因组DNA的分离
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7
Non-methylated CpG-rich islands at the human alpha-globin locus: implications for evolution of the alpha-globin pseudogene.人类α-珠蛋白基因座处富含非甲基化CpG的岛：对α-珠蛋白假基因进化的影响。
EMBO J. 1987 Apr;6(4):999-1004. doi: 10.1002/j.1460-2075.1987.tb04851.x.
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A mouse-human hybrid cell panel for mapping human chromosome 16.
Ann Genet. 1986;29(4):235-9.
9
Anonymous DNA probes to human chromosome 16 derived from a flow-purified library.源自流式纯化文库的人16号染色体匿名DNA探针。
Am J Hum Genet. 1988 Feb;42(2):373-9.
10
A 10-megabase physical map of human Xp21, including the Duchenne muscular dystrophy gene.人类Xp21的一个10兆碱基物理图谱，包括杜氏肌营养不良基因。
Genomics. 1988 Apr;2(3):189-202. doi: 10.1016/0888-7543(88)90002-x.