Zwierzak I, Baleani M, Viceconti M
Laboratorio di Tecnologia Medica, Istituto Ortopedico Rizzoli, Bologna, Italy.
Proc Inst Mech Eng H. 2009 Oct;223(7):913-8. doi: 10.1243/09544119JEIM634.
The hardness of cortical human bone has been measured on osteons in different conditions. However, no data are reported in the literature regarding the effect of cortical tissue condition and indentation location on the measured hardness values. This study aimed to investigate whether the hardness of the human cortical bone evaluated by micro-indentation is influenced, first, by the tissue condition and, second, by the distance of the indentation from the edge of the Haversian canal. Two femura were collected from a subject without musculoskeletal disease. The Vickers hardness was measured by means of microindentation (applied load, 100 gf) on osteons with a cross-section greater than 200 microm. The tests were performed on wet and embedded tissue at different distances from the Haversian canal edge (30-150 microm). No significant differences were found in hardness values between the two contralateral femura. Embedded tissue was significantly harder (12 per cent) than wet tissue. No significant differences were found in hardness values measured at different distances from the Haversian canal edge except for those closer than 60 microm. Therefore, indentations cannot be performed on osteons small in cross-section, since the distance from the closer pore has to be controlled. They should be performed on wet tissue, to avoid an offset in the measured hardness.
已在不同条件下对人皮质骨的骨单位进行了硬度测量。然而，文献中未报告有关皮质组织状况和压痕位置对测量硬度值影响的数据。本研究旨在调查通过微压痕评估的人皮质骨硬度是否首先受组织状况影响，其次受压痕距哈弗斯管边缘距离的影响。从一名无肌肉骨骼疾病的受试者身上采集了两根股骨。通过微压痕（施加载荷100 gf）对横截面大于200微米的骨单位测量维氏硬度。在距哈弗斯管边缘不同距离（30 - 150微米）的湿组织和包埋组织上进行测试。在两根对侧股骨之间未发现硬度值有显著差异。包埋组织比湿组织明显更硬（12%）。除了距哈弗斯管边缘小于60微米处测量的硬度值外，在距哈弗斯管边缘不同距离处测量的硬度值未发现显著差异。因此，不能对横截面小的骨单位进行压痕，因为必须控制距最近孔隙的距离。应在湿组织上进行压痕，以避免测量硬度出现偏差。
