Rho J Y, Zioupos P, Currey J D, Pharr G M
Department of Biomedical Engineering, University of Memphis, TN 38152, USA.
Bone. 1999 Sep;25(3):295-300. doi: 10.1016/s8756-3282(99)00163-5.
The nanoindentation method was used to examine variations in the individual thick lamellar properties within completed secondary osteons as a function of distance from the osteonal center (haversian canal). In general, there is a decline in both elastic modulus and hardness from the center of the osteon outward. Because some of the osteons may have a different general trend than others, an analysis of covariance was also carried out. The overall analysis was highly significant for both elastic modulus and hardness. Also, osteon number was significant as a factor, indicating that there was some difference in the overall thick lamellar properties of the different osteons. An unpaired t-test showed statistically significant differences (p = 0.0005 and 0.0004, respectively) between thick lamellar properties obtained from most of the inner two osteonal lamellae (E = 20.8 +/- 1.3 GPa and H = 0.65 +/- 0.06 GPa) and those from outermost two osteonal lamellae (E = 18.8 +/- 1.0 GPa and H = 0.55 +/- 0.05 GPa). In general, lamellar properties from near to the center of the osteon were greater than those from the outermost osteonal lamella. The mechanical properties of osteons are also significantly lower than those of the interstitial bone (p < 0.0001). The ratio (E1/E2) of the elastic moduli of the outermost osteonal lamella (E1) (considered to be the soft part of the osteons) and that of interstitial bone (E2) was approximately 0.7. These results may have important implications for the mechanical contribution of individual osteons to bone biomechanics.
采用纳米压痕法研究了完整二级骨单位内单个厚板层特性随距骨单位中心（哈弗斯管）距离的变化。一般来说，从骨单位中心向外，弹性模量和硬度均呈下降趋势。由于一些骨单位可能与其他骨单位有不同的总体趋势，因此还进行了协方差分析。总体分析对弹性模量和硬度均具有高度显著性。此外，骨单位数量作为一个因素具有显著性，表明不同骨单位的总体厚板层特性存在一些差异。非配对t检验显示，从最内侧两层骨单位板层获得的厚板层特性（E = 20.8±1.3 GPa，H = 0.65±0.06 GPa）与最外侧两层骨单位板层获得的厚板层特性（E = 18.8±1.0 GPa，H = 0.55±0.05 GPa）之间存在统计学显著差异（p分别为0.0005和0.0004）。一般来说，靠近骨单位中心的板层特性大于最外侧骨单位板层的特性。骨单位的力学性能也显著低于骨间质（p < 0.0001）。最外侧骨单位板层（E1）（被认为是骨单位的软质部分）与骨间质（E2）的弹性模量之比（E1/E2）约为0.7。这些结果可能对单个骨单位对骨生物力学的力学贡献具有重要意义。
